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Document S1. Supplemental Experimental Procedures, One Table, and Two FiguresxDownload (1.25 MB ) Document S1. Supplemental… Expand Large proteins are usually expressed in a eukaryotic system while smaller ones are expressed in prokaryotic systems. For proteins… Expand Preparations enriched by a specific protein are rarely easily obtained from natural host cells. Hence, recombinant protein… Expand Cultivated mammalian cells have become the dominant system for the production of recombinant proteins for clinical applications… Expand The methylotrophic yeast Pichia pastoris is now one of the standard tools used in molecular biology for the generation of… Expand Elastin-like polypeptides (ELPs) undergo a reversible, inverse phase transition. Below their transition temperature (Tt), ELPs… Expand Recombinant proteins containing four cysteines at the i, i + 1, i + 4, and i + 5 positions of an alpha helix were fluorescently… Expand SUMMARY Various gram-negative animal and plant pathogens use a novel, sec-independent protein secretion system as a basic… Expand We have purified and characterized active recombinant human bone morphogenetic protein (BMP) 2A. Implantation of the recombinant… Expand We describe a general purification method for recombinant proteins based upon the selective interaction between a poly-histidine… Expand