Synchronized Formation and Remodeling of Postsynaptic Densities: Long-Term Visualization of Hippocampal Neurons Expressing Postsynaptic Density Proteins Tagged with Green Fluorescent Protein
@article{Ebihara2003SynchronizedFA, title={Synchronized Formation and Remodeling of Postsynaptic Densities: Long-Term Visualization of Hippocampal Neurons Expressing Postsynaptic Density Proteins Tagged with Green Fluorescent Protein}, author={Tatsuhiko Ebihara and Izumi Kawabata and Shinichi Usui and Kenji Sobue and Shigeo Okabe}, journal={The Journal of Neuroscience}, year={2003}, volume={23}, pages={2170 - 2181}, url={https://api.semanticscholar.org/CorpusID:17868397} }
The presence of signaling mechanisms that can induce synchronized addition or elimination of PSD proteins throughout dendritic arborization of a single neuron is suggested.
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42 References
Rapid Redistribution of the Postsynaptic Density Protein PSD-Zip45 (Homer 1c) and Its Differential Regulation by NMDA Receptors and Calcium Channels
- 2001
Biology
D-Zip45 showed rapid redistribution and a higher steady-state turnover rate and neuronal activity differentially redistributes a specific subset of PSD proteins, which are important for localization of both surface receptors and intracellular signaling complexes.
Continual remodeling of postsynaptic density and its regulation by synaptic activity
- 1999
Biology
Continual PSD turnover and its regulation by synaptic activity may be important in activity-dependent remodeling of neuronal connections.
Spine Formation and Correlated Assembly of Presynaptic and Postsynaptic Molecules
- 2001
Biology
The local and stepwise assembly of synaptic components at the contact sites between dendritic protrusions and axons explains the developmental remodeling of excitatory synapses.
Regulation of Dendritic Spine Morphology by SPAR, a PSD-95-Associated RapGAP
- 2001
Biology, Medicine
PSD-95 involvement in maturation of excitatory synapses.
- 2000
Biology
It is found that overexpression of PSD-95 in hippocampal neurons can drive maturation of glutamatergic synapses and this results demonstrate that PSd-95 can orchestrate synaptic development and are suggestive of roles for PSD -95 in synapse stabilization and plasticity.
Assembly of presynaptic active zones from cytoplasmic transport packets
- 2000
Biology
Time-lapse fluorescence imaging, immunocytochemistry and electron microscopy are used to study hippocampal neuronal cultures transfected with a fusion construct of the presynaptic vesicle protein VAMP and green fluorescent protein to suggest that substantial membrane remodeling may be necessary to produce the uniform vesicles typical of the mature active zone.
Rapid spine delivery and redistribution of AMPA receptors after synaptic NMDA receptor activation.
- 1999
Biology
Tetanic synaptic stimulation induced a rapid delivery of tagged receptors into dendritic spines as well as clusters in dendrites and may contribute to the enhanced AMPA receptor-mediated transmission observed during long-term potentiation and activity-dependent synaptic maturation.
Rapid dendritic morphogenesis in CA1 hippocampal dendrites induced by synaptic activity.
- 1999
Biology
High-frequency focal synaptic stimulation induced a period of enhanced growth of small filopodia-like protrusions in dendritic regions close to the stimulating electrode and was prevented by blockade of N-methyl-D-aspartate receptors.
Rapid, Activation-Induced Redistribution of Ionotropic Glutamate Receptors in Cultured Hippocampal Neurons
- 1999
Biology
It is concluded that ionotropic glutamate receptors are regulated in native neurons by rapid, subtype-specific membrane trafficking, which may modulate synaptic transmission in response to physiological or pathophysiological activation.