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Evidence for a Role of Dendritic Filopodia in Synaptogenesis and Spine Formation
It is suggested that dendritic filopodia may actively initiate synaptogenic contacts with nearby axons and thereafter evolve into dendrite spines. Expand
Unitary Assembly of Presynaptic Active Zones from Piccolo-Bassoon Transport Vesicles
Quantitative analysis reveals that the Bassoon, Piccolo, and RIM content of individual PTVs is, on average, half of that of individual presynaptic boutons and shows that the synaptic content of these molecules can be quantitatively accounted for by incorporation of integer numbers (typically two to three) of P TVs into presynptic membranes. Expand
Assembling the Presynaptic Active Zone A Characterization of an Active Zone Precursor Vesicle
The results show that Piccolo is transported to nascent synapses on an approximately 80 nm dense core granulated vesicle together with other constituents of the active zone, including Bassoon, Syntaxin, SNAP-25, and N-cadherin, as well as chromogranin B. Expand
Assembly of New Individual Excitatory Synapses Time Course and Temporal Order of Synaptic Molecule Recruitment
It is suggested that glutamatergic synapse assembly can occur within 1-2 hr after initial contact and that presynaptic differentiation may precede postsynaptic differentiation. Expand
Cellular and molecular mechanisms of presynaptic assembly
The formation of a presynaptic neurotransmitter secretion site involves the transformation of a patch of unspecialized plasma membrane into a complex structure that is highly specialized forExpand
Metabolic Turnover of Synaptic Proteins: Kinetics, Interdependencies and Implications for Synaptic Maintenance
It was found that nearly all synaptic proteins identified here exhibited half-lifetimes in the range of 2–5 days, and metabolic turnover rates were not significantly different for presynaptic and postsynaptic proteins, or for proteins for which mRNAs are consistently found in dendrites. Expand
Local Sharing as a Predominant Determinant of Synaptic Matrix Molecular Dynamics
Findings indicate that the dynamics of key synaptic matrix molecules may be dominated by local protein exchange and redistribution, whereas protein synthesis and degradation serve to maintain and regulate the sizes of local, shared pools of these proteins. Expand
Molecular mechanisms of CNS synaptogenesis
This work has shown that the structure, functional and developmental assembly of glutamatergic synapses of the mammalian CNS, which are asymmetric sites of cell-cell adhesion between nerve cells, are similar to those of the Tournaisian nervous system. Expand
Potentiation of Evoked Vesicle Turnover at Individually Resolved Synaptic Boutons
It is found that brief tetanic stimuli induce a potentiation of evoked synaptic vesicle turnover that lasts for at least 1 hr, and appears to occur approximately equally at boutons of low and high initial release probabilities, and evidently does not involve an increase in the size of the total recycling synapticvesicle pool. Expand
Long-Term Relationships between Synaptic Tenacity, Synaptic Remodeling, and Network Activity
Long term time-lapse imaging reveals that individual synapses undergo significant structural remodeling not only when driven by activity, but also when network activity is absent, raising questionsExpand