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Ultra-sensitive fluorescent proteins for imaging neuronal activity
TLDR
A family of ultrasensitive protein calcium sensors (GCaMP6) that outperformed other sensors in cultured neurons and in zebrafish, flies and mice in vivo are developed and provide new windows into the organization and dynamics of neural circuits over multiple spatial and temporal scales. Expand
Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators
TLDR
A single-wavelength GCaMP2-based GECI (GCaMP3) is developed, with increased baseline fluorescence, increased dynamic range and higher affinity for calcium, and long-term imaging in the motor cortex of behaving mice revealed large fluorescence changes in imaged neurons over months. Expand
ScanImage: Flexible software for operating laser scanning microscopes
TLDR
This work describes a simple, software-based approach to operating a laser scanning microscope without the need for custom data acquisition hardware and quantifies the effectiveness of the data acquisition and signal conditioning algorithm under a variety of conditions. Expand
Optimization of a GCaMP Calcium Indicator for Neural Activity Imaging
TLDR
GCaMP5 fluorescence provides a more reliable measure of neuronal activity than its predecessor GCaMP3, which allows more sensitive detection of neural activity in vivo and may find widespread applications for cellular imaging in general. Expand
Transient and Persistent Dendritic Spines in the Neocortex In Vivo
TLDR
In 6-month-old mice, spines turn over more slowly in visual compared to somatosensory cortex, possibly reflecting differences in the capacity for experience-dependent plasticity in these brain regions. Expand
The subcellular organization of neocortical excitatory connections
TLDR
An efficient channelrhodopsin-2 (ChR2)-assisted method to map the spatial distribution of synaptic inputs, defined by presynaptic ChR2 expression, within the dendritic arborizations of recorded neurons is developed. Expand
The Life Cycle of Ca2+ Ions in Dendritic Spines
TLDR
The kinetics of Ca(2+) sources governs the time course of [Ca(2+)] signals and may explain the selective activation of long-term synaptic potentiation (LTP) and long- term depression (LTD) by NMDA-R-mediated synaptic Ca( 2+). Expand
Long-term in vivo imaging of experience-dependent synaptic plasticity in adult cortex
TLDR
The measurements suggest that sensory experience drives the formation and elimination of synapses and that these changes might underlie adaptive remodelling of neural circuits. Expand
Experience-dependent structural synaptic plasticity in the mammalian brain
TLDR
Recent evidence for structural forms of synaptic plasticity in the mammalian cortex involves cell type-specific structural plasticity: some boutons and dendritic spines appear and disappear, accompanied by synapse formation and elimination, respectively. Expand
A toolbox of Cre-dependent optogenetic transgenic mice for light-induced activation and silencing
TLDR
The robust, consistent and inducible nature of the ChR2 mice represents a significant advance over previous lines, and the Arch-ER2 and eNpHR3.0 mice are to the authors' knowledge the first demonstration of successful conditional transgenic optogenetic silencing. Expand
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