Evidence for a Role of Dendritic Filopodia in Synaptogenesis and Spine Formation

@article{Ziv1996EvidenceFA,
  title={Evidence for a Role of Dendritic Filopodia in Synaptogenesis and Spine Formation},
  author={N. Ziv and Stephen J. Smith},
  journal={Neuron},
  year={1996},
  volume={17},
  pages={91-102}
}
Axo-dendritic synaptogenesis was examined in live hippocampal cell cultures using the fluorescent dyes DiO to label dendrites and FM 4-64 to label functional presynaptic boutons. As the first functional synaptic boutons appeared in these cultures, numerous filopodia (up to 10 micron long) were observed to extend transiently (mean lifetime 9.5 min) from dendritic shafts. With progressively increasing numbers of boutons, there were coincident decreases in numbers of transient filopodia and… Expand
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