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Regulated transcription controls the diversity, developmental pathways and spatial organization of the hundreds of cell types… Expand We have mapped and quantified mouse transcriptomes by deeply sequencing them and recording how frequently each gene is… Expand Mammalian promoters can be separated into two classes, conserved TATA box–enriched promoters, which initiate at a well-defined… Expand MOTIVATION
Promoter analysis is an essential step on the way to identify regulatory networks. A prerequisite for successful… Expand The regulation of cell growth and survival can be subverted by a variety of genetic defects that alter transcriptional programs… Expand Tumor necrosis factor alpha (TNF alpha) is a potent immunomodulator and proinflammatory cytokine that has been implicated in the… Expand Control elements of the tetracycline-resistance operon encoded in Tn10 of Escherichia coli have been utilized to establish a… Expand The RNA polymerase gene of bacteriophage T7 has been cloned into the plasmid pBR322 under the inducible control of the lambda PL… Expand We have developed a procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate… Expand Two hybrid promoters that are functional in Escherichia coli have been constructed. These hybrid promoters, tacI and tacII, were… Expand