beta-galactosidase activity

Replicative senescence limits the proliferation of somatic cells passaged in culture and may reflect cellular aging in vivo. The… (More)

We describe a high-throughput procedure for measuring beta-galactosidase activity in bacteria. This procedure is unique because… (More)

A procedure is described which allows the rapid permeabilization of yeast cells, Schizosaccharomyces pombe and Saccharomyces… (More)

Mice and cells lacking the epidermal growth factor receptor (EGFR) were generated to examine its physiological role in vivo… (More)

Myxococcus xanthus strains containing transcriptional fusions to lacZ were analyzed and fractionated by differences in their… (More)

A general strategy for selecting insertion mutations in mice has been devised. Constructs lacking a promoter and including a beta… (More)

An automated kinetic assay for beta-galactosidase activity in Escherichia coli was developed to permit the measurement of many… (More)

To gain insights into transcription factors defining neuronal identity, we generated transgenic mice carrying a 1.8 kb rat neuron… (More)

The construction and use of a Tn3-lac transposon, Tn3-HoHo1, is described. Tn3-HoHo1 can serve as a transposon mutagen and… (More)

A hybrid gene that consists of the Drosophila heat shock gene, hsp70, fused to the E. coli beta-galactosidase gene has been… (More)