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Nitrogen regulation in Saccharomyces cerevisiae.
The historical foundations of the study of nitrogen regulation as well as the current understanding of the regulatory networks that underlie nitrogen regulation are discussed.
Transcription of glnA in E. coli is stimulated by activator bound to sites far from the promoter
It is shown that the ability of this promoter to be activated by a low intracellular concentration of NRI depends on two binding sites for NRI located approximately 110 and 140 bp, respectively, upstream of the start of transcription.
Control site location and transcriptional regulation in Escherichia coli.
The regulatory regions for 119 Escherichia coli promoters have been analyzed, and the locations of the regulatory sites have been cataloged. The following observations emerge. (i) More than 95% of
Biochemical parameters of glutamine synthetase from Klebsiella aerogenes
The glutamine synthetase from Klebsiella aerogenes is similar to that from Escherichia coli in several respects, but it is necessary to use special methods to harvest growing cells to prevent changes in the adenylylation state of GS from occurring during harvesting.
Covalent modification of the glnG product, NRI, by the glnL product, NRII, regulates the transcription of the glnALG operon in Escherichia coli.
  • A. Ninfa, B. Magasanik
  • Biology, Medicine
    Proceedings of the National Academy of Sciences…
  • 1 August 1986
It is proposed that NRI-phosphate activates transcription from nitrogen-regulated promoters and that the role of NRII is control of the formation and breakdown of N RI-ph phosphate in response to cellular signals of nitrogen availability.
Initiation of transcription at the bacterial glnAp2 promoter by purified E. coli components is facilitated by enhancers
The initiation of transcription from the nitrogen-regulated promoter glnAp2 requires RNA polymerase containing sigma 54, the transcriptional activator NRI, and the protein kinase NRII, responsible
Role of the GATA factors Gln3p and Nil1p of Saccharomyces cerevisiae in the expression of nitrogen-regulated genes.
The NIL1 gene is isolated by virtue of the homology of its zinc-finger domain to that of the previously identified activator, the product of GLN3, and the results show that the nature of GATAAG sequence that serve as the upstream activation sequence elements for these genes determines their abilities to respond to Gln3p and Nil1p.
Transcriptional and posttranslational regulation of the general amino acid permease of Saccharomyces cerevisiae
Results suggest that inactivation of the permease results from its dephosphorylation, which is followed by slower disappearance of the protein.
The URE2 gene product of Saccharomyces cerevisiae plays an important role in the cellular response to the nitrogen source and has homology to glutathione s-transferases.
Study with disruption alleles of the URE2 and GLN3 genes showed that both genes regulate GLN1 and GDH2, the structural genes for glutamine synthetase and NAD-linked glutamate dehydrogenase, respectively, at the transcriptional level, but expression of the regulatory genes does not appear to be regulated.
Role of glnB and glnD gene products in regulation of the glnALG operon of Escherichia coli
The results confirm that only the products of glnF and glnG are essential for this regulation of the synthesis of glutamine synthetase and of histidase in response to nitrogen deprivation or excess.