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Abstract. Five types of zymogens of pepsins, gastric digestive proteinases, are known: pepsinogens A, B, and F, progastricsin… Expand Many species of yeast secrete significant amounts of protease(s). In this article, results of numerous surveys of yeast… Expand We have developed the yeast Kluyveromyces lactis as a host organism for the production of the milk-clotting enzyme chymosin. In… Expand We have studied the expression of a bovine chymosin cDNA in Trichoderma reesei to test the ability of this novel fungal host… Expand The size distribution and density of two types of protein inclusion bodies arising from the synthesis in E. coli of recombinant… Expand Recent reports have shown that synthesis of certain recombinant proteins in Escherichia coli results in the production of… Expand Recombinant calf prochymosin synthesized in E. coli was shown to accumulate in the form of insoluble inclusion bodies. Isolation… Expand We have constructed a high-efficiency expression vector to direct the synthesis of heterologous polypeptides in yeast. The vector… Expand A gene for calf prochymosin (prorennin) has been reconstructed from chemically synthesized oligodeoxyribonucleotides and cloned… Expand Activation of prochymosin at pH below 2.5 results in formation of the active enzyme pseudochymosin by proteolytic cleavage of the… Expand