...................................................................................................................................
xiii Chapter
1
Introduction
..........................................................................................................
1 Background
.........................................................................................................................................
1 Curacin
A
..............................................................................................................................................
1 Biosynthesis
of
natural
products
.................................................................................................
3 Biosynthesis
of
curacin
A
.............................................................................................................................
5 Terminal
double
bond
offloading
.............................................................................................................
5 Canonical
sulfotransferases
and
thioesterases
......................................................................
7 Other
instances
of
ACP-‐ST-‐TE
tridomains
................................................................................
8 Chemistry
related
to
ST-‐TE
olefin
production
.....................................................................
13 ST-‐TE
olefin
offloading
as
a
chemical
tool
.............................................................................
14 The
lost
haloalkane
dehalogenase
CurN/DmmA
................................................................
15 Thesis
overview
..............................................................................................................................
15 Chapter
2
Terminal
Alkene
Formation
by
the
Thioesterase
of
Curacin
A Biosynthesis:
Structure
of
a
Decarboxylating
Thioesterase
..................................
17 Summary
...........................................................................................................................................
17 Introduction
.....................................................................................................................................
18 Experimental
Procedures
...........................................................................................................
21 Crystallization.
...............................................................................................................................................
22 Results
...............................................................................................................................................
26 Discussion
.........................................................................................................................................
39 Conclusion.
......................................................................................................................................................
42