Polymerase Chain Reaction

Known as: Reaction, Polymerase Chain, polymerase chain reaction (PCR), PCR 
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short… (More)

Topic mentions per year

Topic mentions per year

1945-2018
050001000019452017

Papers overview

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Review
2016
Review
2016
Mutation of the KRAS oncogene in pancreatic cancer is responsible for permanent activation of the P21 RAS protein and the cascade… (More)
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Highly Cited
2003
Highly Cited
2003
Quantification of mRNAs using real-time polymerase chain reaction (PCR) by monitoring the product formation with the fluorescent… (More)
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Highly Cited
1993
Highly Cited
1993
We describe a new molecular approach to analyzing the genetic diversity of complex microbial populations. This technique is based… (More)
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Highly Cited
1992
Highly Cited
1992
Effective methods are needed to identify and isolate those genes that are differentially expressed in various cells or under… (More)
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Highly Cited
1991
Highly Cited
1991
The 5'----3' exonuclease activity of the thermostable enzyme Thermus aquaticus DNA polymerase may be employed in a polymerase… (More)
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Highly Cited
1989
Highly Cited
1989
Overlap extension represents a new approach to genetic engineering. Complementary oligodeoxyribonucleotide (oligo) primers and… (More)
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Highly Cited
1989
Highly Cited
1989
Interspersed DNA elements of the form (dC-dA)n.(dG-dT)n constitute one of the most abundant human repetitive DNA families. We… (More)
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Highly Cited
1989
Highly Cited
1989
We report a rapid and sensitive method for the detection of base changes in given sequences of genomic DNA. This technique is… (More)
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Highly Cited
1988
Highly Cited
1988
A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction. The enzyme… (More)
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Highly Cited
1988
Highly Cited
1988
A method is presented for the rapid in vitro amplification of DNA sequences that flank a region of known sequence. The method… (More)
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