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Hydroperoxide metabolism in mammalian organs.
The assay of catalases and peroxidases.
Publisher Summary This chapter discusses the assay of catalases and peroxidases are: (1) catalase assay by disappearance of peroxide; (2) method for crude cell extracts; (3) direct spectrophotometric… Expand
ASSAY OF CATALASES AND PEROXIDASES, IN METHODS IN ENZYMOLOGY
The mitochondrial generation of hydrogen peroxide. General properties and effect of hyperbaric oxygen.
1. Pigeon heart mitochondria produce H(2)O(2) at a maximal rate of about 20nmol/min per mg of protein. 2. Succinate-glutamate and malate-glutamate are substrates which are able to support maximal… Expand
Non-invasive optical spectroscopy and imaging of human brain function
Brain activity is associated with changes in optical properties of brain tissue. Optical measurements during brain activation can assess haemoglobin oxygenation, cytochrome-c-oxidase redox state, and… Expand
The cellular production of hydrogen peroxide.
1. The enzyme-substrate complex of yeast cytochrome c peroxidase is used as a sensitive, specific and accurate spectrophotometric H(2)O(2) indicator. 2. The cytochrome c peroxidase assay is suitable… Expand
Time resolved reflectance and transmittance for the non-invasive measurement of tissue optical properties.
When a picosecond light pulse is incident on biological tissue, the temporal characteristics of the light backscattered from, or transmitted through, the sample carry information about the optical… Expand
Recovery from exercise-induced desaturation in the quadriceps muscles of elite competitive rowers.
- B. Chance, M. T. Dait, C. Zhang, T. Hamaoka, F. Hagerman
- Chemistry, Medicine
- The American journal of physiology
- 1 March 1992
A simple muscle tissue spectrophotometer is adapted to measure the recovery time (TR) for hemoglobin/myoglobin (Hb/Mb) desaturation in the capillary bed of exercising muscle, termed a deoxygenation… Expand
Validation of near-infrared spectroscopy in humans.
- D. Mancini, L. Bolinger, H. Li, K. Kendrick, B. Chance, J. Wilson
- Chemistry, Medicine
- Journal of applied physiology
- 1 December 1994
Near-infrared (NIR) spectroscopy is a noninvasive technique that uses the differential absorption properties of hemoglobin to evaluate skeletal muscle oxygenation. Oxygenated and deoxygenated… Expand
The respiratory chain and oxidative phosphorylation.
The respiratory chain and oxidative phosphorylation are of utter importance for a cell’s metabolism. Here, the energy won from ingested food is being converted into our body’s energy unit (adenosine… Expand