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Nested Polymerase Chain Reaction

Known as: Nested PCR, PCR, Nested 
Polymerase chain reaction that uses two sets of primers, in two sequential runs of amplification, with the second set intended to amplify a target… 
National Institutes of Health

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Polymerase Chain Reaction

Papers overview

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2013
2013
Development of RT-PCR and Nested PCR for Detecting Four Quarantine Plant Viruses Belonging to Nepovirus
For quarantine purpose, we developed the RT- and nested PCR module of Tomato black ring virus (TBRV), Arabis mosaic virus (ArMV… 
2009
2009
Molecular Identification of the Light Brown Apple Moth (Lepidoptera: Tortricidae) in California Using a Polymerase Chain Reaction Assay of the Internal Transcribed Spacer 2 Locus
ABSTRACT A molecular protocol using a hemi-nested polymerase chain reaction (PCR) of the internal transcribed spacer region 2… 
2006
2006
[Comparative studies on detection of Chlamydophila psittaci and Chlamydophila abortus in meat turkey flocks using cell culture, ELISA, and PCR].
The prevalence of chlamydia in 10 meat turkey flocks was investigated. As samples served of each moment of collection and sex of… 
2001
2001
DEVELOPMENT OF A NESTED PCR PROTOCOL FOR DETECTION OF OLIVE-INFECTING VIRUSES IN CRUDE EXTRACTS
A reverse transcription nested polymerase chain reaction (RT-n-PCR) was developed and applied to the detection of Arabis mosaic… 
1998
1998
Relationship between HBV viremia level of pregnant women and intrauterine infection:neated PCR for detection of HBV DNA.
AIM:To determine the incidence of hepatitis B virus (HBV) in trauterine infection and to explore the relationship between HBV… 
1997
1997
Molecular subtyping of human T‐lymphotropic virus type I (HTLV‐I) by a nested polymerase chain reaction‐restriction fragment length polymorphism analysis of the envelope gene: Two distinct lineages…
The major type of human T‐lymphotropic virus type I (HTLV‐I), generally referred to as the cosmopolitan type, has been grouped… 
1997
1997
Value of the preoperative detection of prostate-specific-antigen-positive circulating cells by nested RT-PCR in patients submitted to radical prostatectomy.
OBJECTIVE To assess the value of the detection of circulating prostate cells [prostate-specific antigen (PSA) positive] by… 
1996
1996
Is hepatitis C virus-RNA detection by nested polymerase chain reaction clinically relevant in hemodialysis patients?
We have prospectively studied in hemodialysis (HD) patients the evolution of hepatitis C virus (HCV) viremia and the putative… 
1995
1995
Detection of human papillomavirus DNA in primary lung carcinoma by nested polymerase chain reaction.
Human papillomaviruses (HPV) have been implicated in the pathogenesis of human squamous cell carcinoma, especially of cervical… 
Review
1993
Review
1993
Direct polymerase chain reaction for detection of human immunodeficiency virus in blood spot residues on filter paper after elution of antibodies: an adjunct to serological surveys for estimating…
Blood spot residues on filter paper circles from which antibodies have been eluted remain suitable for follow-up polymerase chain…