Pamine
National Institutes of Health
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中药活性成分的快速分离分析一直是中药研究的重点和热点问题。该文建立了在线亲和固相萃取-高效液相色谱-二极管阵列检测-四极杆飞行时间质谱技术快速筛选中药中与 α -葡萄糖苷酶有结合作用的活性成分的方法。四通阀和六通进样阀组成接口界面,收集亲和固相萃取柱中与 α -葡萄糖苷酶有结合作用的活性成分,接着进入液相色谱系统进行分析鉴定。阳性对照品((+)-儿茶素)和阴性对照品(水杨酸)混合物筛选确定了方法的特异性和准确性。接着,以玉竹为研究对象,筛选出9种主要的 α -葡萄糖苷酶抑制活性化合物,包括5种苯乙基肉桂酰胺类化合物,4种双氢高异黄酮类化合物。结果表明建立的方法简便、快速、特异性强,可用于任何复杂体系中 α -葡萄糖苷酶结合活性成分的筛选。
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mesolimbic dopamine system consisting of dopaminergic neurons projecting from the ventral tegmental
area (VTA) to the…
Sex, drugs, and other pleasurable stimuli activate reward circuits in the midbrain. The ventral tegmental area (VTA) and nucleus…
The purpose of the present series of tests was to measure and compare the effects of ingestion of gelatin capsules containing 15…
We modified the single-isotope radioenzymatic assay for catecholamines [Life Sci. 21, 625 (1977)] to assay 3, 4…
grossesse compliquee d'anurie semble frequente en Jamaique. Dans 5 des 6 cas, une diminution soudaine et prolongee de la pression…
"Peptic ulcer is the product of a pathological process as a result of which the gastroduodenal mucosa fails to withstand the…