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Sepsis remains a prevalent clinical challenge and the underlying pathophysiology is still poorly understood. To investigate the… Expand Cellular processes can only be understood as the dynamic interplay of molecules. There is a need for techniques to monitor… Expand We describe a new method for relative quantification of 40 different DNA sequences in an easy to perform reaction requiring only… Expand In just a few short years, the chemical ligation of unprotected peptide segments in aqueous solution has established itself as… Expand A protein semisynthesis method-expressed protein ligation-is described that involves the chemoselective addition of a peptide to… Expand A novel DNA fingerprinting technique called AFLP is described. The AFLP technique is based on the selective PCR amplification of… Expand A simple technique has been devised that allows the direct synthesis of native backbone proteins of moderate size. Chemoselective… Expand We attempted to develop an experimental animal model for peripheral neuropathic pain. Under sodium pentobarbital anesthesia, both… Expand A new procedure has been developed for the efficient cloning of complex PCR mixtures, resulting in libraries exclusively… Expand