Antagonistic Role of H-NS and GadX in the Regulation of the Glutamate Decarboxylase-dependent Acid Resistance System in Escherichia coli*

@article{Giangrossi2005AntagonisticRO,
  title={Antagonistic Role of H-NS and GadX in the Regulation of the Glutamate Decarboxylase-dependent Acid Resistance System in Escherichia coli*},
  author={Mara Giangrossi and Stefano Zattoni and Angela Tramonti and Daniela De Biase and Maurizio Falconi},
  journal={Journal of Biological Chemistry},
  year={2005},
  volume={280},
  pages={21498 - 21505},
  url={https://api.semanticscholar.org/CorpusID:24966136}
}
Evidence is provided that H-NS directly inhibits gadA and gadX transcription and, by controlling the intracellular level of the activator GadX, indirectly affects the expression of the whole gad system.
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It is shown that repression of hdeAB by MarA depends on pH, growth phase, and other regulators of h deAB and is associated with reduced resistance to acid conditions.

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Polyamines Are Critical for the Induction of the Glutamate Decarboxylase-dependent Acid Resistance System in Escherichia coli *

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Integration Host Factor is Required for the Induction of Acid Resistance in Escherichia coli

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45 References

Transcriptional Expression of Escherichia coli Glutamate-Dependent Acid Resistance Genes gadA and gadBC in an hns rpoS Mutant

It is demonstrated that gad transcription can be detected in an hns rpoS mutant strain of E. coli K-12, indicating that gad promoters can be initiated by sigma(70) in the absence of H-NS.

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ABSTRACT An important feature of Escherichia coli pathogenesis is an ability to withstand extremely acidic environments of pH 2 or lower. This acid resistance property contributes to the low…

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Cluster and principal-component analyses identified 15 GadX-regulated, acid-inducible genes and reverse transcriptase mapping demonstrated that these genes are organized in 10 operons, and it was confirmed that GadX is a transcriptional activator under acidic growth conditions.

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Structural analysis of this chromosomal region suggests that the promoters of the corresponding genes are preferentially denatured, supporting the existence of a fitness island for acid adaptation on the E. coli chromosome.

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