.................................................................................................................................... 0 Table of contents ....................................................................................................................... 2 Abbreviation list ....................................................................................................................... 3 Introduction .............................................................................................................................. 4 1. Background ......................................................................................................................... 4 2. B-cells and the lymphatic system ........................................................................................ 4 3. Mantle cell lymphoma ........................................................................................................ 5 4. Endocannabinoid system .................................................................................................... 6 a. Other possible interacting receptors .............................................................................. 6 b. Ligands ........................................................................................................................... 6 c. Hemopressin ................................................................................................................... 7 5. Cell death pathways ............................................................................................................ 7 Aim ............................................................................................................................................. 9 Materials and Methods .......................................................................................................... 10 1. Reagents ............................................................................................................................ 10 2. Instruments ....................................................................................................................... 10 3. Softwares .......................................................................................................................... 10 4. Cell culture ....................................................................................................................... 10 5. Freezing and thawing of cells ........................................................................................... 10 6. Viability assay ................................................................................................................... 11 7. Protein lysate preparation and protein concentration estimation ................................... 11 8. Western blotting ................................................................................................................ 11 Results ...................................................................................................................................... 12 1. CB1 & CB2 receptor expression ...................................................................................... 12 2. CB1 and CB2 antagonists’ effects on cell viability .......................................................... 14 3. Agonists effect on cell viability ......................................................................................... 15 4. Detection of apoptosis and autophagy ............................................................................. 16 5. GPR55 receptor expression .............................................................................................. 18 6. GPR55 agonist effect on cell viability .............................................................................. 19 7. Effect of Anandamide in cells pretreated with antagonists .............................................. 20 8. Combined effect of CB1 and CB2 antagonists ................................................................. 22 Discussion ................................................................................................................................ 23 References ................................................................................................................................ 25