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Soft Agar Assay

Soft agar colony formation assay is performed to assay for anchorage independent growth.
National Institutes of Health

Papers overview

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2015
2015
Subcellular Localization and Ser-137 Phosphorylation Regulate Tumor-suppressive Activity of Profilin-1*
Background: The actin-binding protein profilin-1 is a eukaryotic protein essential for growth, with poorly understood antitumor… 
2011
2011
Integrin-linked kinase regulates phosphatase and tensin homologue activity to promote tumorigenesis in neuroblastoma cells.
2010
2010
TSG101, identified by screening a cancer cDNA library and soft agar assay, promotes cell proliferation in human lung cancer
Understanding the genesis and development of tumors is an essential component in cancer research. It is of interest to discover… 
2005
2005
1-(5-Chloro-2-alkoxyphenyl)-3-(5-cyanopyrazin-2-yl)ureas [correction of cyanopyrazi] as potent and selective inhibitors of Chk1 kinase: synthesis, preliminary SAR, and biological activities.
The discovery of 1-(5-chloro-2-alkoxyphenyl)-3-(5-cyanopyrazin-2-yl)ureas as a new class of potent (IC(50) values of 3-10 nM) and… 
2004
2004
Clonal growth of bladder cancer cells in a double layer soft agar assay: needles addition of multiple culture supplements
SummaryThe necessity of multiple culture supplements, characteristic of Hamburger and Salmon (H&S) soft agar assay (1977), for… 
1991
1991
A serum-free medium for the Courtenay-Mills soft agar assay.
A serum-free medium has been developed to support the clonogenic growth in soft agar of human cervical carcinoma cell lines… 
1986
1986
Impact Of Metastases on Nodal Immunoreactivity in Head and Neck Cancer
It has been previously demonstrated by the authors that lymph nodes from patients with head and neck cancer are capable of… 
1983
1983
Culture of clonogenic cells from various human tumors: drug sensitivity assay.
1982
1982
Colony-forming cell proliferation: a rapid and sensitive assay system for murine granulocyte and macrophage colony-stimulating factors.
A microculture assay for murine granulocyte-macrophage colony-stimulating factor (GM-CSF) has been developed using fetal liver GM… 
1981
1981
Cultivation of human melanomas in soft agar. Factors influencing plating efficiency and chemosensitivity
As part of a programme to study the predictive clinical value of a soft agar assay for measuring chemosensitivity of human… 
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