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Reverse Transcriptase Polymerase Chain Reaction

Known as: RT PCR, Polymerase Chain Reaction, Reverse Transcriptase, PCR, Reverse Transcriptase 
A laboratory procedure in which an RNA strand is first transcribed into a DNA complement and then subjected to PCR amplification. Transcribing an RNA… Expand
National Institutes of Health

Related topics

Related topics

6 relations
Clinical Data Interchange Standards Consortium TerminologyRNA-Directed DNA PolymeraseReverse Transcriptionstandards characteristics

Broader (2)

Polymerase Chain ReactionTranscription, Genetic

Papers overview

Semantic Scholar uses AI to extract papers important to this topic.
Review
2019
Review
2019
ESMO recommendations on the standard methods to detect NTRK fusions in daily practice and clinical research.
NTRK1, NTRK2 and NTRK3 fusions have been reported in a plethora of malignancies across different histologies and represent the… Expand
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Highly Cited
2013
Highly Cited
2013
STAR: ultrafast universal RNA-seq aligner
MOTIVATION Accurate alignment of high-throughput RNA-seq data is a challenging and yet unsolved problem because of the non… Expand
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Review
2004
Review
2004
Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction.
Polymerase chain reaction (PCR)-based assays can target either DNA (the genome) or RNA (the transcriptome). Targeting the genome… Expand
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Highly Cited
2004
Highly Cited
2004
A multigene assay to predict recurrence of tamoxifen-treated, node-negative breast cancer.
BACKGROUND The likelihood of distant recurrence in patients with breast cancer who have no involved lymph nodes and estrogen… Expand
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Review
2003
Review
2003
Standardization and quality control studies of ‘real-time’ quantitative reverse transcriptase polymerase chain reaction of fusion gene transcripts for residual disease detection in leukemia – A…
Detection of minimal residual disease (MRD) has proven to provide independent prognostic information for treatment stratification… Expand
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Highly Cited
2002
Highly Cited
2002
Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR.
Real-time reverse transcription followed by polymerase chain reaction (RT-PCR) is the most suitable method for the detection and… Expand
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Highly Cited
2001
Highly Cited
2001
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
The two most commonly used methods to analyze data from real-time, quantitative PCR experiments are absolute quantification and… Expand
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Highly Cited
1999
Highly Cited
1999
Development and validation of real-time quantitative reverse transcriptase-polymerase chain reaction for monitoring gene expression in cardiac myocytes in vitro.
In this article we present validation of a real-time RT-PCR method to quantitate mRNA expression levels of atrial natriuretic… Expand
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Highly Cited
1997
Highly Cited
1997
Reverse transcriptase motifs in the catalytic subunit of telomerase.
Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Telomerase RNA… Expand
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Highly Cited
1992
Highly Cited
1992
Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction.
We report on the development and application of a rapid assay for detecting and typing dengue viruses. Oligonucleotide consensus… Expand
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