Reverse Transcriptase Polymerase Chain Reaction

Known as: RT PCR, Polymerase Chain Reaction, Reverse Transcriptase, PCR, Reverse Transcriptase 
A laboratory procedure in which an RNA strand is first transcribed into a DNA complement and then subjected to PCR amplification. Transcribing an RNA… (More)
National Institutes of Health

Topic mentions per year

Topic mentions per year

1968-2019
020004000600019682019

Related topics

Related topics

6 relations
Clinical Data Interchange Standards Consortium TerminologyPolymerase Chain ReactionRNA-Directed DNA PolymeraseReverse Transcription
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Broader (1)

Transcription, Genetic

Related mentions per year

Related mentions per year

1936-2019
19401960198020002020
Reverse Transcriptase Polymerase Chain Reaction
Transcription, Genetic
standards characteristics
Polymerase Chain Reaction
Reverse Transcription
RNA-Directed DNA Polymerase

Papers overview

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Highly Cited
2009
Highly Cited
2009
Determination of mRNA expression of human UDP-glucuronosyltransferases and application for localization in various human tissues by real-time reverse transcriptase-polymerase chain reaction.
An exhaustive real-time reverse transcriptase-polymerase chain reaction (PCR) quantification method was used to determine 15 of… (More)
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Highly Cited
2004
Highly Cited
2004
Measurement of gene expression in archival paraffin-embedded tissues: development and performance of a 92-gene reverse transcriptase-polymerase chain reaction assay.
Throughout the last decade many laboratories have shown that mRNA levels in formalin-fixed and paraffin-embedded (FPE) tissue… (More)
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Highly Cited
2002
Highly Cited
2002
Comparative detection of lymph node micrometastases of stage II colorectal cancer by reverse transcriptase polymerase chain reaction and immunohistochemistry.
PURPOSE Inconsistent conclusions have been drawn about the clinical significance of micrometastases in lymph nodes (LNs) of node… (More)
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Highly Cited
2001
Highly Cited
2001
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
The two most commonly used methods to analyze data from real-time, quantitative PCR experiments are absolute quantification and… (More)
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Highly Cited
2000
Highly Cited
2000
Significant detection of circulating cancer cells in the blood by reverse transcriptase-polymerase chain reaction during colorectal cancer resection.
OBJECTIVE To analyze the clinical value of reverse transcriptase-polymerase chain reaction (RT-PCR) recognition of mRNA coding… (More)
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Highly Cited
1999
Highly Cited
1999
Development and validation of real-time quantitative reverse transcriptase-polymerase chain reaction for monitoring gene expression in cardiac myocytes in vitro.
In this article we present validation of a real-time RT-PCR method to quantitate mRNA expression levels of atrial natriuretic… (More)
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Highly Cited
1997
Highly Cited
1997
Limitations of reverse-transcriptase polymerase chain reaction analyses for detection of micrometastatic epithelial cancer cells in bone marrow.
PURPOSE This study was designed to evaluate the potential of reverse-transcriptase polymerase chain reaction (RT-PCR) analyses… (More)
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Highly Cited
1995
Highly Cited
1995
Detection of cancer micrometastases in lymph nodes by reverse transcriptase-polymerase chain reaction.
There are few DNA-based studies that detect cancer micrometastases in lymph nodes. We have assayed for the specific detection of… (More)
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Highly Cited
1994
Highly Cited
1994
Sensitive detection of occult breast cancer by the reverse-transcriptase polymerase chain reaction.
PURPOSE Detection of occult carcinoma in patients with breast cancer may aid the establishment of prognosis and development of… (More)
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Highly Cited
1992
Highly Cited
1992
Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction.
We report on the development and application of a rapid assay for detecting and typing dengue viruses. Oligonucleotide consensus… (More)
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