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Studying membrane proteins represents a major challenge in protein biochemistry, with one of the major difficulties being the… Expand Detergents are indispensable in the isolation of integral membrane proteins from biological membranes to study their intrinsic… Expand There is a need to standardize the NDF procedure. Procedures have varied because of the use of different amylases in attempts to… Expand Bicinchoninic acid, sodium salt, is a stable, water-soluble compound capable of forming an intense purple complex with cuprous… Expand A rapid method based on a defined methanol-chloroform-water mixture for the quantitative precipitation of soluble as well as… Expand Some recent modifications of the protein assay by the method of Lowry, Rosebrough, Farr, and Randall (1951, J. Biol. Chem.193… Expand Abstract A protein determination method which involves the binding of Coomassie Brilliant Blue G-250 to protein is described. The… Expand This review focuses on work that deals with the mechanisms of detergent action in membrane solubilization including properties of… Expand A rapid procedure for determining cellwall constituents of plants consista of the determination of the fiber insoluble in neutral… Expand The presence in mammalian liver microsomes of a carbon monoxide-binding pigment has been reported by Klingenberg (1) and by… Expand