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Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
RNA interference (RNAi) is the process of sequence-specific, post-transcriptional gene silencing in animals and plants, initiated by double-stranded RNA (dsRNA) that is homologous in sequence to theExpand
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The organization of titin filaments in the half-sarcomere revealed by monoclonal antibodies in immunoelectron microscopy: a map of ten nonrepetitive epitopes starting at the Z line extends close to
mAbs specific for titin or nebulin were characterized by immunoblotting and fluorescence microscopy. Immunoelectron microscopy on relaxed chicken breast muscle revealed unique transverse stripingExpand
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Analysis of gene function in somatic mammalian cells using small interfering RNAs.
RNA interference (RNAi) is a highly conserved gene silencing mechanism that uses double-stranded RNA (dsRNA) as a signal to trigger the degradation of homologous mRNA. The mediators ofExpand
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Identification of essential genes in cultured mammalian cells using small interfering RNAs.
We report the first RNAi-induced phenotypes in mammalian cultured cells using RNA interference mediated by duplexes of 21-nt RNAs. The 21 gene products studied have different functions andExpand
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Post-translational modifications regulate microtubule function
The αβ-tubulin heterodimer, the building block of microtubules, is subject to a large number of post-translational modifications, comparable in diversity to the intensively studied histoneExpand
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Essential roles for Caenorhabditis elegans lamin gene in nuclear organization, cell cycle progression, and spatial organization of nuclear pore complexes.
Caenorhabditis elegans has a single lamin gene, designated lmn-1 (previously termed CeLam-1). Antibodies raised against the lmn-1 product (Ce-lamin) detected a 64-kDa nuclear envelope protein.Expand
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Effect of Clathrin Heavy Chain- and α-Adaptin-specific Small Inhibitory RNAs on Endocytic Accessory Proteins and Receptor Trafficking in HeLa Cells*
To assess the contribution of individual endocytic proteins to the assembly of clathrin coated pits, we depleted the clathrin heavy chain and the α-adaptin subunit of AP-2 in HeLa-cells using RNAExpand
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Immunofluorescence and immunocytochemical procedures with affinity purified antibodies: tubulin-containing structures.
Publisher Summary This chapter describes the study of the intracellular location of proteins by immunocytochemical procedures. Although the methods given in the chapter are generally applicable, theyExpand
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Sequence, chemical, and structural variation of small interfering RNAs and short hairpin RNAs and the effect on mammalian gene silencing.
Small interfering RNAs (siRNAs) induce sequence-specific gene silencing in mammalian cells and guide mRNA degradation in the process of RNA interference (RNAi). By targeting endogenous lamin A/C mRNAExpand
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Measurement of molecular weights by electrophoresis on SDS-acrylamide gel.
Publisher Summary This chapter describes methods for sodium dodecyl sulfate (SDS) gel electrophoresis and the characterization of proteins separated on SDS gels. SDS dissociates proteins into theirExpand
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