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Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
21-nucleotide siRNA duplexes provide a new tool for studying gene function in mammalian cells and may eventually be used as gene-specific therapeutics. Expand
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
  • K. Weber, M. Osborn
  • Medicine, Chemistry
  • The Journal of biological chemistry
  • 25 August 1969
The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins. Expand
The organization of titin filaments in the half-sarcomere revealed by monoclonal antibodies in immunoelectron microscopy: a map of ten nonrepetitive epitopes starting at the Z line extends close to
Tin molecules appear as polar structures lacking over large regions repetitive epitopes over half-sarcomere lengths, and one physical end seems related to Z line anchorage, while the other may bind close to the M line. Expand
Analysis of gene function in somatic mammalian cells using small interfering RNAs.
Because of the robustness of the siRNA knockdown technology, genomewide analysis of human gene function in cultured cells has now become possible and here a collection of protocols for siRNA-mediated knockdown of mammalian gene expression is provided. Expand
Identification of essential genes in cultured mammalian cells using small interfering RNAs.
The first RNAi-induced phenotypes in mammalian cultured cells using RNA interference mediated by duplexes of 21-nt RNAs are reported, and two other lamins, B1 and B2, are now identified as essential proteins. Expand
Post-translational modifications regulate microtubule function
The αβ-tubulin heterodimer, the building block of microtubules, is subject to a large number of post-translational modifications, comparable in diversity to the intensively studied histoneExpand
Essential roles for Caenorhabditis elegans lamin gene in nuclear organization, cell cycle progression, and spatial organization of nuclear pore complexes.
Observations show that lmn-1 is an essential gene in C. elegans, and that the nuclear lamins are involved in chromatin organization, cell cycle progression, chromosome segregation, and correct spacing of NPCs. Expand
Immunofluorescence and immunocytochemical procedures with affinity purified antibodies: tubulin-containing structures.
This chapter describes the study of the intracellular location of proteins by immunocytochemical procedures, and the methods given are generally applicable for tubulin containing structures and antibodies to tubulin. Expand
Golgi-derived membranes that contain an acylated viral polypeptide are used for vaccinia virus envelopment
A 37,000-dalton polypeptide present on purified extracellular vaccinia virus but absent from intracellular virus particles of classical morphology was further characterized and showed that it is relatively hydrophobic. Expand