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Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
21-nucleotide siRNA duplexes provide a new tool for studying gene function in mammalian cells and may eventually be used as gene-specific therapeutics.
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
Intermediate filaments: structure, dynamics, function, and disease.
Analysis of gene function in somatic mammalian cells using small interfering RNAs.
The organization of titin filaments in the half-sarcomere revealed by monoclonal antibodies in immunoelectron microscopy: a map of ten nonrepetitive epitopes starting at the Z line extends close to…
Tin molecules appear as polar structures lacking over large regions repetitive epitopes over half-sarcomere lengths, and one physical end seems related to Z line anchorage, while the other may bind close to the M line.
Identification of essential genes in cultured mammalian cells using small interfering RNAs.
- J. Harborth, S. Elbashir, K. Bechert, T. Tuschl, K. Weber
- BiologyJournal of Cell Science
- 15 December 2001
The first RNAi-induced phenotypes in mammalian cultured cells using RNA interference mediated by duplexes of 21-nt RNAs are reported, and two other lamins, B1 and B2, are now identified as essential proteins.
Post-translational modifications regulate microtubule function
The αβ-tubulin heterodimer, the building block of microtubules, is subject to a large number of post-translational modifications, comparable in diversity to the intensively studied histone…
Essential roles for Caenorhabditis elegans lamin gene in nuclear organization, cell cycle progression, and spatial organization of nuclear pore complexes.
Observations show that lmn-1 is an essential gene in C. elegans, and that the nuclear lamins are involved in chromatin organization, cell cycle progression, chromosome segregation, and correct spacing of NPCs.
Immunofluorescence and immunocytochemical procedures with affinity purified antibodies: tubulin-containing structures.