Base Sequence

High-throughput sequencing assays such as RNA-Seq, ChIP-Seq or barcode counting provide quantitative readouts in the form of… (More)

We present Model-based Analysis of ChIP-Seq data, MACS, which analyzes data generated by short read sequencers such as Solexa's… (More)

We have mapped and quantified mouse transcriptomes by deeply sequencing them and recording how frequently each gene is… (More)

'BLAST 2 Sequences', a new BLAST-based tool for aligning two protein or nucleotide sequences, is described. While the standard… (More)

The identification of potential regulatory motifs in new sequence data is increasingly important for experimental design. Those… (More)

A novel DNA fingerprinting technique called AFLP is described. The AFLP technique is based on the selective PCR amplification of… (More)

Gene splicing by overlap extension is a new approach for recombining DNA molecules at precise junctions irrespective of… (More)

We report the complete thermodynamic library of all 10 Watson-Crick DNA nearest-neighbor interactions. We obtained the relevant… (More)

Three kinds of improvements have been introduced into the M13-based cloning systems. (1) New Escherichia coli host strains have… (More)

A new method for determining nucleotide sequences in DNA is described. It is similar to the "plus and minus" method [Sanger, F… (More)