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More than other analytical procedures, immunoassays have afforded a wealth of knowledge about biochemical physiology over the… Expand Fluorogenic substrates based on 4-methylumbelliferone (4-MU) have been widely used for the detection of phosphatase and… Expand A fluorescent enzyme inhibition assay for okadaic acid using 4-methylumbelliferyl phosphate and fluorescein diphosphate as… Expand To clarify its physiologic role, alkaline phosphatase (ALP) was examined in normal skin fibroblasts and was shown to be the… Expand The phylogenetic relationships of 30 taxa of Holarctic hylid frogs were examined using starch gel electrophoresis. Allelic… Expand A continuous spectrofluorimetric assay of calmodulin-dependent phosphatase is described. The assay monitors the formation of a… Expand Leishmania donovani, the causative agent in kala-azar or visceral leishmaniasis, infects cells of the macrophage system. We show… Expand We describe a new assay that is useful for identifying individuals who may be affected with Gaucher's disease. The assay involves… Expand 1. The kinetics of inhibition of calf-intestinal alkaline phosphatase by inorganic phosphate, fluorophosphate, inorganic… Expand 1. The effects of varying pH, ionic strength and temperature on the parameters K(m) and V(max.) for a purified alkaline… Expand