New enzyme-linked immunosorbent assay for glycocalicin in plasma.
@article{Kunishima1991NewEI, title={New enzyme-linked immunosorbent assay for glycocalicin in plasma.}, author={Shinji Kunishima and K Hayashi and S. Kobayashi and Tomoki Naoe and Ryuzo Ohno}, journal={Clinical chemistry}, year={1991}, volume={37 2}, pages={ 169-72 }, url={https://api.semanticscholar.org/CorpusID:6738650} }
It is concluded that this assay is suitable for measuring glycocalicin in plasma and is also more sensitive and precise than the previously published immunoassays based on competitive binding assay.
27 Citations
Glycocalicin: a new assay--the normal plasma levels and its potential usefulness in selected diseases.
- 1994
Medicine
The results of patients with aplastic anemia and thrombocytosis confirmed that GC levels are clearly dependent on the platelet count, and the proposed GC index suggest that GC is a useful platelet marker in certain diseases, which directly reflects platelet damage and possibly platelet dysfunction.
Rapid Detection of Plasma Glycocalicinby a Latex Agglutination Test and Its Use in the Differential Diagnosis of Thrombocytopaenia
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The aim of this study was to develop a rapid assay for glycocalicin which could be used in the differential diagnosis of thrombocytopaenia.
Serum thrombopoietin and plasma glycocalicin concentrations as useful diagnostic markers in thrombocytopenic disorders
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Medicine
Measurements of TPO and glycocalicin levels are useful for the diagnosis of thrombocytopenia, and the results from ITP patients did not support the model which suggested the simple feedback regulation of T PO in thromBocy topenia.
Increase in Glycocalicin Levels in Platelet Concentrates Stored in Plasma or Synthetic Medium for 8 Days: Comparison with Other Platelet Activation Markers
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Medicine
The GC concentration correlates well with sensitive platelet (activation) parameters, rendering it a sensitive and convenient parameter for platelet activation.
Increased but highly dispersed levels of plasma glycocalicin in patients with disseminated intravascular coagulation
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In patients with DIC, thrombocytopenia is not always due to increased platelet consumption, but it may be due to decreased production of platelets, suggesting bone marrow insufficiency in these patients.
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Glycocalicin in the diagnosis and management of immune thrombocytopenia
- 1998
Medicine
The data confirm that the GC index is helpful in differentiating thrombocytopenia due to increased platelet destruction from the one due to impaired production and the assay has been proven useful in the differential diagnosis of different ITP subtypes and their follow-up.
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27 References
Evidence that glycocalicin circulates in normal plasma.
- 1984
Biology, Medicine
There appears to be ongoing catabolism of platelet GPIb in vivo, and it is postulate that the plasma level of glycocalicin reflects a complex function of factors, including platelet count, platelet turnover, and the site of platelets and platelet destruction.
Platelet glycocalicin. Interaction with thrombin and role as thrombin receptor of the platelet surface.
- 1978
Biology, Medicine
Thrombin-binding activity of platelets remained under conditions where platelet aggregating ability is lost and the amount of thrombin bound by modified platelets was proportional to the total amount of glycocalicinlglycoprotein I present.
Platelet glycocalicin. Its membrane association and solubilization in aqueous media.
- 1980
Biology, Chemistry
Utilization of the biotin/avidin system to amplify the sensitivity of the enzyme-linked immunosorbent assay (ELISA).
- 1983
Chemistry, Medicine
The use of avidin-biotin interaction in immunoenzymatic techniques.
- 1979
Chemistry
Biotin was covalently attached to antibodies, antigens and enzymes, and the effects of this labeling on the antigen and antibody binding capacity and on enzymatic activity were tested. Based on…
Plasma glycocalicin. An aid in the classification of thrombocytopenic disorders.
- 1987
Medicine
It is indicated that the measurement of plasma glycocalicin may be a useful adjunct in classifying thrombocytopenic disorders.
Relationship between glycocalicin and glycoprotein lb of human platelets ( Factor VIII-related von Willebrand factor receptor / platelet glycoproteins / peanut agglutinin affinity chromatography / radioactive peptide map )
Biology, Chemistry
Evidence is presented that the platelet receptor for bovine von Willebrand factor is an integral membrane glycoprotein that differs from glycocalicin in binding strongly to phenyl-Sepharose, indicating that it contains a hydrophobic moiety while glycocicin does not.
Purification and preliminary characterization of the glycoprotein Ib complex in the human platelet membrane.
- 1985
Biology, Medicine
Human platelet glycoprotein Ib is a major integral membrane protein that has been identified as the platelet-binding site mediating the factor VIII/von Willebrand-factor-dependent adhesion of platelets to vascular subendothelium and both GP Ib and GP IX were found to occur in the same immunoprecipitin arc whether the platelets had been solubilized in the absence or presence of the calcium-dependent protease inhibitor, leupeptin.