Isolation and characterization of two group A rotaviruses with unusual genome profiles.

@article{Pocock1987IsolationAC,
  title={Isolation and characterization of two group A rotaviruses with unusual genome profiles.},
  author={D. H. Pocock},
  journal={The Journal of general virology},
  year={1987},
  volume={68 ( Pt 3)},
  pages={
          653-60
        },
  url={https://api.semanticscholar.org/CorpusID:24394665}
}
Analysis of the genomic dsRNA of rotaviruses isolated from calves with subclinical infections has revealed eight calves excreting group A viruses with unusual genome profiles, which are similar to those of the bovine group A rotavirus UK strain.

Figures from this paper

Genome rearrangements in porcine rotaviruses: biochemical and biological comparisons between a supershort strain and its standard counterpart.

Results suggest that the rearranged strain was probably generated from the standard one and that the coding capacity of the rearrange segment was conserved, and the possibility that large plaque formation and efficient virus replication can be influenced by the products of genomic segment 11, in addition to segment 4, is discussed.

Molecular characterization of three rabbit rotavirus strains

Sequence analysis of the individual RNA segments will confirm whether genome rearrangements are the molecular basis for these novel migration patterns.

Molecular and Serologic Characterization of a Group a Bovine Rotavirus with a Short Genome Pattern

The serologic and molecular characterization (G and P specificity) of a bovine rotavirus strain, 2292B, with a short genome pattern is described and the possibility that BRV short pattern isolates are restricted to serotype G6, similar to the situation observed for human G2 rotaviruses is investigated.

Heterogeneity of genome rearrangements in rotaviruses isolated from a chronically infected immunodeficient child

Rotaviruses with genome rearrangements, isolated from a chronically infected immunodeficient child, were adapted to growth in BSC-1 cells, andome rearrangement is proposed to be a third mechanism directing the evolution of rotavirus evolution.

Isolation and characterization of a new simian rotavirus, YK-1

A new simian rotavirus, designated YK-1, was isolated from a 2-year-old immunodeficient pigtailed macaque with chronic diarrhea and is being used to establish a nonhuman primate model for studying the infectivity, immunity, and pathogenesis ofRotavirus and for evaluating candidate rotav virus vaccines.

Partial characterization of a bovine group A rotavirus with a short genome electropherotype

A group A rotavirus (ID isolate) recovered from a diarrheic beef calf possessed a short genome electropherotype, which was a stable characteristic of the isolate as it remained unchanged through 3 passages in gnotobiotic calves or through 19 passages in MA104 cell cultures.

Rearrangements of Rotavirus Genomic Segment 11 Are Generated during Acute Infection of Immunocompetent Children and Do Not Occur at Random

It is shown that under such conditions of infection, the viral RNA polymerase generates rearrangements which occur not at random but within direct repeats which might constitute hot spots for RNA recombination.

Rearrangement of genomic segment 11 in two swine rotavirus strains.

Analysis of protein profiles of normal and rearranged strains showed that the gene product of segment 11 had no apparent change in its relative electrophoretic migration, suggesting that the rearranged genes remained functional.

Rearrangement of the VP6 gene of a group A rotavirus in combination with a point mutation affecting trimer stability

The degree of relatedness of VP6 of the Chinese lamb rotavirus Lp14 to those of other group A rotaviruses was determined and the nucleotide and predicted amino acid sequences of normal and rearranged RNA 6, coding for the major inner capsid protein VP6, were reported.

Rotavirus gene structure and function.

Detailed analyses with recently characterized immunologic and gene probes and new animal models can be expected to provide a basic understanding of what regulates the primary interactions of these viruses with the gastrointestinal tract and the subsequent responses of infected hosts.

Molecular characterization of rotaviruses with distinct group antigens.

Experimental criteria have been established which allow the division of rotaviruses into three groups and it is indicated that the viruses in the three groups did not carry any genome segments in common with each other.

Definition of two new groups of atypical rotaviruses.

Comparative antigenic and nuclei acid analyses were carried out on two new atypical rotavirus isolates coming respectively from chickens and pigs, indicating that all of their genomic RNAs had significant differences in sequence both from each other and from the three previously defined rotav virus groups.

Comparison of the genomes of simian, bovine, and human rotaviruses by gel electrophoresis and detection of genomic variation among bovine isolates

By co-electrophoresis in polyacrylamide gels, the segmented double-standed RNA genome of the simian rotavirus, SA 11, was compared with those of human and bovine rotaviruses and a scheme for the systematic designation of different rotav virus samples is proposed.

Molecular biology of rotaviruses. I. Characterization of basic growth parameters and pattern of macromolecular synthesis

Comparison of the infected-cell polypeptides with those present in purified virions revealed that mot of the virus-induced proteins were incorporated into virions, with only VP9 being a truly nonstructural protein.

Reassortment of human rotaviruses carrying rearranged genomes with bovine rotavirus.

Upon superinfection with the tissue culture-adapted UK Compton strain of bovine rotavirus (BRV) extensive genome reassortment occurred, and reassortant viruses possessed functional proteins coded for by the genome segments and/or by rearranged bands of RNA of the human rotaviruses.

In vitro transcription and translation of simian rotavirus SA11 gene products

Three of the viral structural polypeptides (VP4, -5, and -5a) were not synthesized in vitro as primary gene products, demonstrating that processing must occur for the production of some structural polyPEptides.

The genomes of rotaviruses isolated from chronically infected immunodeficient children.

Hybridization studies using segment-specific cloned cDNA probes indicated that some of the additional bands found migrating between RNA segments had sequence homologies with normal rotavirus dsRNA segments.