........................................................................................................................................... i Acknowledgements ........................................................................................................................ iv Table of Content ............................................................................................................................. v List of Figures ............................................................................................................................... vii List of Tables .................................................................................................................................. x Chapter 1 Introduction .............................................................................................................. 1 1.1 General .................................................................................................................................. 1 1.2 Development of optical chemical sensing device for diabetes monitoring ........................... 1 1.3 Resorcinol and tiglic acid imbedded membrane for acetone detection in high relative humidity. ................................................................................................................................... 3 1.4 Resorcinol and tiglic acid imbedded membrane for acetone detection in high relative humidity. ................................................................................................................................... 5 1.5 Optical sensing device for real time acetone detection ......................................................... 6 1.6 Objective of Study ................................................................................................................. 7 Chapter 2 Materials and Methodology ..................................................................................... 9 2.1 Membrane Preparation .......................................................................................................... 9 2.1.1 12g/L resorcinol imbibed membrane preparation .......................................................... 9 2.1.2 Acid based membrane preparation ................................................................................. 9 2.2 Experimental Methods ........................................................................................................ 10 2.2.1 Tiglic acid concentration calibration ............................................................................ 10 2.2.1.1 Absorbance determination of different concentration of tiglic acid in ethanol ..... 10 2.2.1.2 Absorbance determination of different concentration of tiglic acid in Nafion membrane ........................................................................................................................... 10 2.2.2 Acetone exposure experiments ..................................................................................... 10 2.2.2.1 12g/L resorcinol imbibed membrane ..................................................................... 10 2.2.2.2 Different concentration of acid based membrane .................................................. 11 2.2.3 Flow cell acetone exposure experiments ...................................................................... 11 2.2.4 Second Generation CRMAD prototype for real time acetone detection ...................... 12 2.2.4.1 Different relative humidity detection ..................................................................... 15 2.2.4.2 Different flow rate detection .................................................................................. 15