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sequenase activity

Known as: sequenase 
National Institutes of Health

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DNA-directed DNA polymerase activity

Papers overview

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1998
1998
Effect of single benzo[a]pyrene diol epoxide-deoxyguanosine adducts on the action of DNA polymerases in vitro.
Neither Sequenase 2.0 nor Klenow fragment were able to extend 12-mer primers using the eight templates (16-mers) derived by… 
1997
1997
Sequence context is an important determinant in the mutagenic potential of 1,N6-ethenodeoxyadenosine (epsilonA): formation of epsilonA basepairs and elongation in defined templates.
Many laboratories have obtained data on mutagenicity of modified bases in naturally occurring DNA sequences. It has often been… 
1994
1994
Primer extension by various polymerases using oligonucleotide templates containing stereoisomeric benzo[a]pyrene-deoxyadenosine adducts.
Four isomeric benzo[a]pyrene-deoxyadenosine adducts, corresponding to the products of trans opening of the epoxide ring in the… 
1994
1994
Internal fluorescence labeling with fluorescent deoxynucleotides in two-label peak-height encoded DNA sequencing by capillary electrophoresis.
Fluorescently labeled deoxynucleotides were used for internal labeling of DNA sequencing fragments generated in a two-color peak… 
1993
1993
Screening for point mutations by semi-automated DNA sequencing using sequenase and magnetic beads.
We have established an improved method for detecting point mutations by semi-automated DNA sequencing of PCR fragments generated… 
1993
1993
Effect of site-specifically located mitomycin C-DNA monoadducts on in vitro DNA synthesis by DNA polymerases.
A series of site-specifically modified oligodeoxynucleotides were synthesized that contained either of the two known mitomycin C… 
1992
1992
DNA polymerase, RNA polymerase and exonuclease activities on a DNA sequence modified by benzo[a]pyrene diolepoxide.
Adducts produced by modification of DNA with benzo[a]pyrene diolepoxide (BPDE) are known to inhibit both DNA and RNA synthesis… 
1991
1991
Analysis of mutations using PCR and denaturing gradient gel electrophoresis
Denaturing gradient gel electrophoresis (DGGE) separates DNA molecules based on primary sequence. Under the appropriate… 
1990
1990
A method for the determination of sequence of high G+C% DNA by the sequential application of sequenase and Taq polymerase.
In this protocol, both enzymes are used sequentially to minimize the artifacts attributable to polymerase malfunction. Thus, the… 
1988
1988
DNA sequencing using Taq polymerase.
Three DNA polymerases, namely E.coli DNA polymerase 1 (Klenow), reverse transcriptase and T7 DNA polymerase (sequenase), are… 
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