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ribosomal skipping

A translation process in which a specific viral peptide prevents the ribosome from covalently linking a new inserted amino acid, and lets it continue… 
National Institutes of Health

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viral translation

Papers overview

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2020
2020
Simplifying the Detection of Surface Presentation Levels in Yeast Surface Display by Intracellular tGFP Expression.
Yeast surface display (YSD) is an ultra-high throughput method used in protein engineering. Protein-protein interactions as well… 
2019
2019
An optimized yeast display strategy for efficient scFv antibody selection using ribosomal skipping system and thermo resistant yeast
Establish a method to restrict unexpected fragments including stop codons in scFv library and generate a thermo resistant strain… 
2018
2018
A tightly regulated and adjustable CRISPR-dCas9 based AND gate in yeast
Abstract The robust and precise on and off switching of one or more genes of interest, followed by expression or repression is… 
2017
2017
Inefficient Ribosomal Skipping Enables Simultaneous Secretion and Display of Proteins in Saccharomyces cerevisiae.
The need for recombinant expression of soluble protein slows the validation of engineered proteins isolated from combinatorial… 
2017
2017
A simplified procedure for antibody engineering by yeast surface display: Coupling display levels and target binding by ribosomal skipping.
Yeast surface display is a valuable, widely used method for protein engineering. However, current yeast display applications rely… 
2016
2016
Optimized P2A for reporter gene insertion into Nipah virus results in efficient ribosomal skipping and wild-type lethality.
Incorporation of reporter genes within virus genomes is an indispensable tool for interrogation of virus biology and pathogenesis… 
2015
2015
688. AAV8-Mediated Liver Gene Targeting Without Nucleases Rescues Lethality in a Mouse Model of the Crigler-Najjar Syndrome
Highly Cited
2014
Highly Cited
2014
Promoterless gene targeting without nucleases ameliorates haemophilia B in mice
Site-specific gene addition can allow stable transgene expression for gene therapy. When possible, this is preferred over the use… 
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