intein-mediated protein splicing activity

Known as: intein, intein-mediated protein splicing 
The removal of an internal amino acid sequence (an intein) from a protein during protein maturation; the excision of inteins is precise and the N… (More)
National Institutes of Health

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cyclization of asparagine involved in intein-mediated protein splicingcyclization of glutamine involved in intein-mediated protein splicing
endonuclease activityintein

Papers overview

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Review
2005
Review
2005
The mechanism of intein-mediated protein splicing: variations on a theme.
Intein-mediated protein splicing is facilitated by four separate but coordinated nucleophilic displacement reactions that result… (More)
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Review
2005
Review
2005
Intein-mediated protein trans-splicing and transgene containment in plastids.
Transgenes in plastids are contained by stringent maternal inheritance in most cultivated plant species and their expression… (More)
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Highly Cited
2005
Highly Cited
2005
Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies
Segmental isotopic labeling of proteins using protein ligation is a recently established in vitro method for incorporating… (More)
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Highly Cited
2001
Highly Cited
2001
Split luciferase as an optical probe for detecting protein-protein interactions in mammalian cells based on protein splicing.
We describe a new method for detecting protein-protein interactions in intact mammalian cells; the approach is based on protein… (More)
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Review
2001
Review
2001
Intein spread and extinction in evolution.
Inteins are selfish DNA elements found within coding regions. They are translated with their host protein, but then catalyze… (More)
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Highly Cited
2000
Highly Cited
2000
InBase: the Intein Database
Inteins are self-catalytic protein splicing elements. InBase (http://www.neb.com/neb/inteins.html), the Intein Database and… (More)
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Highly Cited
1998
Highly Cited
1998
Protein trans-splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803.
A split intein capable of protein trans-splicing is identified in a DnaE protein of the cyanobacterium Synechocystis sp. strain… (More)
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Highly Cited
1998
Highly Cited
1998
Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step.
A conventional affinity protein purification system often requires a separate protease to separate the target protein from the… (More)
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Highly Cited
1998
Highly Cited
1998
Semisynthesis of cytotoxic proteins using a modified protein splicing element.
Two cytotoxic proteins, bovine pancreatic ribonuclease A (RNase A), and a restriction endonuclease from Haemophilus… (More)
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1998
1998
Control of protein splicing by intein fragment reassembly.
Inteins are protein splicing elements that mediate their excision from precursor proteins and the joining of the flanking protein… (More)
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