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Two-Hybrid System Techniques

Known as: Two Hybrid System Technics, Two-Hybrid System Technique, Methods, Two-Hybrid 
Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between… 
National Institutes of Health

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Blotting, Far-WesternOne-Hybrid System TechniquesThree-Hybrid System TechniquesYeast Three-Hybrid System Techniques

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Yeast Two-Hybrid System Techniques

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Highly Cited
2007
Highly Cited
2007
Retracted: LPA2 Is Required for Efficient Assembly of Photosystem II in Arabidopsis thaliana
Biogenesis and assembly of photosystem II is mainly regulated by nuclear-encoded factors. This work reports the identification of… 
Highly Cited
2005
Highly Cited
2005
2-Cyano-3,12-dioxoolean-1,9-dien-28-oic Acid and Related Compounds Inhibit Growth of Colon Cancer Cells through Peroxisome Proliferator-Activated Receptor γ-Dependent and -Independent Pathways
2-Cyano-3,12-dioxoolean-1,9-dien-28-oic acid (CDDO) and the corresponding methyl (CDDO-Me) and imidazole (CDDO-Im) esters induce… 
Highly Cited
2004
Highly Cited
2004
A new precursor for the immobilization of enzymes inside sol-gel-derived hybrid silica nanocomposites containing polysaccharides.
Highly Cited
2001
Highly Cited
2001
Yeast PalA/AIP1/Alix Homolog Rim20p Associates with a PEST-Like Region and Is Required for Its Proteolytic Cleavage
ABSTRACT The Saccharomyces cerevisiae zinc finger protein Rim101p is activated by cleavage of its C-terminal region, which… 
Highly Cited
2000
Highly Cited
2000
Inhibition of Rainbow Trout (Oncorhynchus mykiss) Estrogen Receptor Activity by Cadmium1
Abstract This study was conducted to determine if the cadmium-mediated inhibition of vitellogenesis observed in fish collected… 
Highly Cited
1998
Highly Cited
1998
Interaction of Mouse Polycomb-Group (Pc-G) Proteins Enx1 and Enx2 with Eed: Indication for Separate Pc-G Complexes
ABSTRACT The Polycomb group (Pc-G) constitutes an important, functionally conserved group of proteins, required to stably… 
Highly Cited
1997
Highly Cited
1997
Human cytomegalovirus capsid assembly protein precursor (pUL80.5) interacts with itself and with the major capsid protein (pUL86) through two different domains
We have used the yeast GAL4 two-hybrid system to examine interactions between the human cytomegalovirus (HCMV) major capsid… 
Highly Cited
1991
Highly Cited
1991
Functional differences between yeast and human TFIID are localized to the highly conserved region
Highly Cited
1977
Highly Cited
1977
Five hundredfold overproduction of DNA ligase after induction of a hybrid lambda lysogen constructed in vitro.
A lambda vector that contains the gene for Escherichia coli DNA ligase (lambdagt4-lop-11 lig+) has been modified to achieve… 
Highly Cited
1966
Highly Cited
1966
Reversible inactivation of dehydrogenases.
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