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Cultivated mammalian cells have become the dominant system for the production of recombinant proteins for clinical applications… Expand Use of the real-time polymerase chain reaction (PCR) to amplify cDNA products reverse transcribed from mRNA is on the way to… Expand TNF and Fas ligand induce apoptosis in tumor cells; however, their severe toxicity toward normal tissues hampers their… Expand Recombinant adenoviruses provide a versatile system for gene expression studies and therapeutic applications. We report herein a… Expand Lewy bodies and Lewy neurites are the defining neuropathological characteristics of Parkinson’s disease and dementia with Lewy… Expand Three kinds of improvements have been introduced into the M13-based cloning systems. (1) New Escherichia coli host strains have… Expand We constructed a series of recombinant genomes which directed expression of the enzyme chloramphenicol acetyltransferase (CAT) in… Expand We describe a new basis for the construction of a genetic linkage map of the human genome. The basic principle of the mapping… Expand A procedure for extracting plasmid DNA from bacterial cells is described. The method is simple enough to permit the analysis by… Expand We describe a technique for transferring electrophoretically separated bands of double-stranded DNA from agarose gels to… Expand