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PNGase F activity

Known as: peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity, N-linked-glycopeptide-(N-acetyl-beta-D-glucosaminyl)-L-asparagine amidohydrolase activity, PNGase F 
Catalysis of the reaction: 4-N-(N-acetyl-D-glucosaminyl)-protein + H2O = N-acetyl-beta-D-glucosaminylamine + peptide L-aspartate. This reaction is… 
National Institutes of Health

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Glycopeptidase F

Papers overview

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Highly Cited
2010
Highly Cited
2010
Tandem 18O stable isotope labeling for quantification of N-glycoproteome.
A new strategy using tandem (18)O stable isotope labeling (TOSIL) to quantify the N-glycosylation site occupancy is reported… 
2006
2006
z-VAD-fmk inhibits peptide:N-glycanase and may result in ER stress
After insertion into the endoplasmic reticulum (ER), many proteins are glycosylated and participate in chaperone-assisted folding… 
2006
2006
Structural and biochemical studies of the C-terminal domain of mouse peptide-N-glycanase identify it as a mannose-binding module
The inability of certain N-linked glycoproteins to adopt their native conformation in the endoplasmic reticulum (ER) leads to… 
Highly Cited
2006
Highly Cited
2006
Immunogenicity of a receptor-binding domain of SARS coronavirus spike protein in mice: Implications for a subunit vaccine
Highly Cited
2005
Highly Cited
2005
Comparative glycoproteomics of N-linked complex-type glycoforms containing sialic acid in human serum.
This study describes a simple and efficient approach for comparative analysis of sialylated glycoforms of proteins containing… 
Highly Cited
2002
Highly Cited
2002
Use of a lectin affinity selector in the search for unusual glycosylation in proteomics.
2001
2001
Chemo-enzymatic synthesis of bioactive glycopeptide using microbial endoglycosidase.
Highly Cited
1999
Highly Cited
1999
Partial glycosylation of Asn2181 in human factor V as a cause of molecular and functional heterogeneity. Modulation of glycosylation efficiency by mutagenesis of the consensus sequence for N-linked…
Coagulation factor V (FV) circulates in two forms, FV1 and FV2, having slightly different molecular masses and phospholipid… 
Highly Cited
1999
Highly Cited
1999
Characterization of carbohydrates using highly fluorescent 2-aminobenzoic acid tag following gel electrophoresis of glycoproteins.
Application of the most sensitive fluorescent label 2-aminobenzoic acid (anthranilic acid, AA) for characterization of… 
Highly Cited
1994
Highly Cited
1994
Functional role of N-glycosylation in alpha 5 beta 1 integrin receptor. De-N-glycosylation induces dissociation or altered association of alpha 5 and beta 1 subunits and concomitant loss of…
Fibronectin (FN)-mediated cell adhesion is controlled mainly by alpha 5 beta 1 (recognizing the RGD sequence) and alpha 4 beta 1… 
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