................................................................................................................................... 1 Declaration ............................................................................................................................... 9 List of tables ........................................................................................................................... 10 Chapter one ....................................................................................................................... 10 Chapter two ....................................................................................................................... 10 Chapter eight ..................................................................................................................... 10 List of figures .......................................................................................................................... 11 Chapter one ....................................................................................................................... 11 Chapter two ....................................................................................................................... 12 Chapter three ..................................................................................................................... 12 Chapter four ....................................................................................................................... 13 Chapter five ........................................................................................................................ 14 Chapter six ......................................................................................................................... 18 Chapter seven .................................................................................................................... 18 Abbreviations ......................................................................................................................... 20 Acknowledgements ................................................................................................................ 28 Dissemination ........................................................................................................................ 29 Chapter 1: Introduction ......................................................................................................... 30 1.1 LDHA ............................................................................................................................. 30 1.1.1 Lactate dehydrogenase (LDH) ............................................................................... 30 1.1.2 The role of LDHA in cellular metabolism .............................................................. 32 1.1.3 Regulation of LDHA synthesis ............................................................................... 34 1.1.4 Post-translational regulation of LDHA .................................................................. 38 1.1.5 The role of LDHA in the cell cycle .......................................................................... 38 1.1.6 LDHA as a transcription factor .............................................................................. 39 1.1.7 Other roles of LDHA .............................................................................................. 40 1.1.8 LDHA in the brain and cerebral spinal fluid .......................................................... 41 1.1.9 LDHA and tumour malignancy .............................................................................. 44 1.1.10 The role of LDHA in tumour growth and survival ............................................... 44 1.1.11 The role of LDHA in tumour migration and metastasis ...................................... 47