His-His-His-His-His-His

Tandem affinity strategies reach exceptional protein purification grades and have considerably improved the outcome of mass… Expand

We aspired to create chemical recognition units, which bind oligohistidine tags with high affinity and stability, as tools for… Expand

AbstractWe have compared four different vectors for expression of proteins with N- or C-terminal hexahistidine (His6) tags in… Expand

When cultured in broth, fresh clinical isolates of the gram-negative periodontal pathogen Actinobacillus actinomycetemcomitans… Expand

An improved method for integration of exogenous DNA fragments at a defined site within the genome of Pseudomonas aeruginosa was… Expand

ABSTRACT The proteasome is a multisubunit protease responsible for degrading proteins conjugated to ubiquitin. The 670-kDa core… Expand

To generate a stable resource from which high affinity human antibodies to any given antigen can be rapidly isolated, functional… Expand

To determine whether the product of the recently cloned ob gene functions as an adipose-related satiety factor, recombinant… Expand

Extracellular signal-regulated protein kinases (ERK) 1 and 2 and mutants of each were expressed in bacteria with a hexahistidine… Expand

Previous work has shown that endogenous chemical mediators, of which histamine is the prototype, increase the permeability of… Expand