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Use of real-time polymerase chain reaction and molecular beacons for the detection of Escherichia coli O157:H7.
Molecular beacons (MBs) are oligonucleotide probes that fluoresce upon hybridization. In this paper, we described the development of a real-time PCR assay to detect the presence of Escherichia coliExpand
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Biodegradation of organophosphorus pesticides by surface-expressed organophosphorus hydrolase
Organophosphorus hydrolase (OPH) was displayed and anchored onto the surface of Escherichia coli using an Lpp-OmpA fusion system. Production of the fusion proteins in membrane fractions was verifiedExpand
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Enhanced bioaccumulation of heavy metals by bacterial cells displaying synthetic phytochelatins.
A novel strategy using synthetic phytochelatins is described for the purpose of developing microbial agents for enhanced bioaccumulation of toxic metals. Synthetic genes encoding for severalExpand
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Engineering Plant-Microbe Symbiosis for Rhizoremediation of Heavy Metals
ABSTRACT The use of plants for rehabilitation of heavy-metal-contaminated environments is an emerging area of interest because it provides an ecologically sound and safe method for restoration andExpand
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Phylogenetic diversity and metabolic potential of activated sludge microbial communities in full-scale wastewater treatment plants.
The activated sludge process is an essential process for treating domestic and industrial wastewaters in most wastewater treatment plants (WWTPs). This process consists of a mixture of general andExpand
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Substrate inhibition kinetics for microbial growth and synthesis of poly-β-hydroxybutyric acid by Alcaligenes eutrophus ATCC 17697
SummaryThe specific growth rate of Alcaligenes eutrophus ATCC 17697 was observed to depend upon the ratio of ammonium sulfate (nitrogen) and fructose (carbon) concentration used for production ofExpand
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Modelling of the acetone‐butanol fermentation with cell retention
A mathematical model for the kinetics of the acetone-butanol fermentation by Clostridium acetobutylicum was developed reflecting the biochemical pathway and culture behavior. Steady-stateExpand
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Nanowire‐Based Electrochemical Biosensors
We review recent advances in biosensors based on one-dimensional (1-D) nanostructure field-effect transistors (FET). Specifically, we address the fabrication, functionalization, assembly/alignmentExpand
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Reversible conversion of conducting polymer films from superhydrophobic to superhydrophilic.
Controlling the wettability of a solid surface is important for myriad applications, ranging from self-cleaning surfaces to microfluidics to biomedicine. Recently, a variety of smart surfaces withExpand
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Enhanced Arsenic Accumulation in Engineered Bacterial Cells Expressing ArsR
ABSTRACT The metalloregulatory protein ArsR, which offers high affinity and selectivity toward arsenite, was overexpressed in Escherichia coli in an attempt to increase the bioaccumulation ofExpand
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