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Enzyme-Linked Immunospot Assay

Known as: ELISPOT, Immunospot Assays, Enzyme-Linked, Immunospot Assay, Enzyme-Linked 
A highly sensitive immunoassay capable of detecting secreted materials from individual cells upon activation. If the material of interest is secreted… 
National Institutes of Health

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Clinical Data Interchange Standards Consortium TerminologyEnzyme-Linked Immunosorbent Assaystandards characteristics

Papers overview

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Highly Cited
2008
Highly Cited
2008
Safety and immunogenicity of recombinant low-dosage HIV-1 A vaccine candidates vectored by plasmid pTHr DNA or modified vaccinia virus Ankara (MVA) in humans in East Africa.
Highly Cited
2007
Highly Cited
2007
Studies of a prophylactic HIV-1 vaccine candidate based on modified vaccinia virus Ankara (MVA) with and without DNA priming: effects of dosage and route on safety and immunogenicity.
Highly Cited
2006
Highly Cited
2006
Modulation of the Immune Response Induced by Gene Electrotransfer of a Hepatitis C Virus DNA Vaccine in Nonhuman Primates
Induction of multispecific, functional CD4+ and CD8+ T cells is the immunological hallmark of acute self-limiting hepatitis C… 
Highly Cited
2002
Highly Cited
2002
Generation of CD8+ T Cell Memory in Response to Low, High, and Excessive Levels of Epitope1
The magnitude of a virus-specific memory CTL population can dramatically influence the outcome of secondary infections, yet… 
Highly Cited
2002
Highly Cited
2002
Design and validation of an enzyme-linked immunospot assay for use in clinical trials of candidate HIV vaccines.
The enzyme-linked immunosorbent (ELISPOT) assay, which enumerates peripheral blood mononuclear cells (PBMCs) releasing interferon… 
Highly Cited
2000
Highly Cited
2000
Quantitation of antigen-reactive T cells in peripheral blood by IFNgamma-ELISPOT assay and chromium-release assay: a four-centre comparative trial.
Highly Cited
1997
Highly Cited
1997
Analysis of the T cell response to tumor and viral peptide antigens by an IFNγ‐ELISPOT assay
We have established a sensitive ELISPOT assay measuring interferon γ (IFN γ) release on a single‐cell basis to detect influenza… 
Highly Cited
1995
Highly Cited
1995
Intratracheal gene delivery with adenoviral vector induces elevated systemic IgG and mucosal IgA antibodies to adenovirus and beta-galactosidase.
One major concern about using adenoviral vectors for repetitive gene delivery to lung epithelial cells is the induction of an… 
Highly Cited
1992
Highly Cited
1992
Reverse ELISPOT assay for clonal analysis of cytokine production. II. Enumeration of interleukin-1-secreting cells by amplified (avidin-biotin anti-peroxidase) assay.
Highly Cited
1985
Highly Cited
1985
Evidence for a Common Mucosal Immune System in Humans
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