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Chromatography, Affinity

Known as: Affinity chromatography, affinity purification, Chromatography, Bioaffinity 
A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in… Expand
National Institutes of Health

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Highly Cited
2005
Highly Cited
2005
Systematic mapping of protein–protein interactions, or ‘interactome’ mapping, was initiated in model organisms, starting with… Expand
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Review
2002
Review
2002
Galectins are widely distributed sugar-binding proteins whose basic specificity for beta-galactosides is conserved by… Expand
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Highly Cited
1999
Highly Cited
1999
We have developed a generic procedure to purify proteins expressed at their natural level under native conditions using a novel… Expand
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Highly Cited
1999
Highly Cited
1999
A novel procedure for micropurification of phosphorylated peptides, as a front end to mass spectrometric analysis, is described… Expand
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Highly Cited
1993
Highly Cited
1993
The N-ethylmaleimide-sensitive fusion protein (NSF) and the soluble NSF attachment proteins (SNAPs) appear to be essential… Expand
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Highly Cited
1988
Highly Cited
1988
Plasmid expression vectors have been constructed that direct the synthesis of foreign polypeptides in Escherichia coli as fusions… Expand
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Highly Cited
1975
Highly Cited
1975
CONVENTIONAL nonspecific precipitation methods sometimes depend on affinities which can be used in a more selective fashion by… Expand
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Highly Cited
1970
Highly Cited
1970
  • P. Cuatrecasas
  • The Journal of biological chemistry
  • 1970
  • Corpus ID: 34804880
Abstract The preparation of a number of agarose and polyacrylamide bead derivatives useful in the purification of proteins by… Expand
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Highly Cited
1970
Highly Cited
1970
Plasminogen was prepared from human plasma by affinity chromatography on L-lysine-substituted Sepharose. Thirty milligrams of… Expand
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Highly Cited
1968
Highly Cited
1968
In this article, Cuatrecasas, Wilchek, and Anfinsen explain the general principles and potential applications of "affinity… Expand
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