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Chromatography, Affinity

Known as: Affinity chromatography, affinity purification, Chromatography, Bioaffinity 
A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in… 
National Institutes of Health

Related topics

Related topics

5 relations
Biotinylationstandards characteristics

Broader (1)

Chromatography

Narrower (2)

Immunoaffinity ChromatographyTAPAL (tandem affinity purification and labeling)

Papers overview

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Highly Cited
1985
Highly Cited
1985
Antibodies to the scrapie protein decorate prion rods.
Scrapie is a degenerative, transmissible neurologic disease of sheep and goats which occurs in the absence of any detectable host… 
Highly Cited
1979
Highly Cited
1979
A novel method of activation of cross-linked agaroses with 1,1'-carbonyldiimidazole which gives a matrix for affinity chromatography devoid of additional charged groups.
1,1'-Carbonyldiimidazole, a carbonylating reagent, has been shown to be suitable for the activation of cross-linked agaroses for… 
Highly Cited
1979
Highly Cited
1979
Purification and characterization of estrogen-binding proteins from calf uterus. 4. Estrogen-binding proteins of calf uterus. Purification to homogeneity of receptor from cytosol by affinity…
: The estrogen receptor has been purified to homogeneity from calf uterus cytosol by sequential affinity chromatography by using… 
Highly Cited
1977
Highly Cited
1977
Activities of lectins and their immobilized derivatives in detergent solutions. Implications on the use of lectin affinity chromatography for the purification of membrane glycoproteins.
The effects of several commonly used detergents on the saccharide-binding activities of lectins were investigated using lectin… 
Highly Cited
1975
Highly Cited
1975
Evaluation of quantitative affinity chromatography by comparison with kinetic and equilibrium dialysis methods for the analysis of nucleotide binding to staphylococcal nuclease.
The elution of staphylococcal nuclease on thymidine 3'-(p-Sepharose-aminophenyl phosphate) 5'-phosphate (nucleotide ligand of… 
Highly Cited
1974
Highly Cited
1974
Preparation of adenosine nucleotide derivatives suitable for affinity chromatography.
Methods of synthesizing a series of chemically-defined AMP, ADP, ATP, adenylyl imidodiphosphate and pyrophosphate derivatives… 
Highly Cited
1974
Highly Cited
1974
Affinity chromatography of kinases and dehydrogenases on Sephadex and Sepharose dye derivatives.
Biospecific affinity chromatography has been shown to be a powerful method for the isolation of biological compounds1,2. While… 
Highly Cited
1973
Highly Cited
1973
Affinity chromatography of nicotinamide-adenine dinucleotide-linked dehydrogenases on immobilized derivatives of the dinucleotide.
1. Three established methods for immobilization of ligands through primary amino groups promoted little or no attachment of NAD… 
Highly Cited
1972
Highly Cited
1972
Affinity chromatography of DNA-binding enzymes on single-stranded DNA-agarose columns.
Agarose gels containing immobilized single-stranded circular DNA from phage fd or denatured calf thymus DNA were investigated for… 
Highly Cited
1970
Highly Cited
1970
The purification of papain by affinity chromatography.
A water insoluble derivative of a papain inhibitor was prepared by covalently linking Gly-Gly-Tyr(Bzl)-Arg to an agarose resin…