Protein Microarray Copying: Easy on‐Demand Protein Microarray Generation Compatible with Fluorescence and Label‐Free Real‐Time Analysis

@article{Kilb2019ProteinMC,
  title={Protein Microarray Copying: Easy on‐Demand Protein Microarray Generation Compatible with Fluorescence and Label‐Free Real‐Time Analysis},
  author={Normann Kilb and Tobias Herz and J{\"u}rgen Burger and Johannes Woehrle and Philipp A. Meyer and G{\"u}nter Roth},
  journal={ChemBioChem},
  year={2019},
  volume={20},
  url={https://api.semanticscholar.org/CorpusID:73426540}
}
A cost‐effective and user‐friendly method for the cell‐free production of protein microarrays from a polydimethylsiloxane flow cell containing an expressible DNA microarray.

How to copy and paste DNA microarrays

A DNA microarray copy technique was developed and was able to copy patterned original DNA microarrays and as a homage to the very first photocopy made by Chester Charlson and Otto Kornei, a lookalike DNA micro arrays were performed exactly 80 years later.

An ultra-high-throughput screen for the evaluation of peptide HLA-Binder interactions

An ultra-high-throughput screening platform named ValidaTe is developed that enables large scale evaluation of pHLA-binder interactions and is illustrated that a massively increased throughput is obtained by the microarray screening, paving the way for use in pre-clinical safety screening of p HLA-targeting drugs.

Direct-Write Bioprinting Approach to Construct Multilayer Cellular Tissues

A novel direct-write bioprinting approach to spatiotemporally position different cellular layers may represent an efficient tool to develop living constructs especially for regeneration of complex flat tissues.

Critical assessment of relevant methods in the field of biosensors with direct optical detection based on fibers and waveguides using plasmonic, resonance, and interference effects

This review will concentrate on a comparison of the optical methods, their application, and the resulting bioanalytical quality.

Protein microarray generation by in situ protein expression from template DNA

A historical timeline of the different methods to generate protein microarrays by cell‐free expression is provided, highlights differences and similarities, and reports the current state of the the different approaches.

Multiplexed Nucleic Acid Programmable Protein Arrays

The developed Multiplexed Nucleic Acid Programmable Protein Array (M-NAPPA) significantly increases the number of displayed proteins by multiplexing as many as five different gene plasmids within a printed spot, demonstrating that multiplexed features on a protein microarray offer a cost-effective fabrication approach and have the potential to facilitate high throughput translational research.

Integrated Method of in Situ Cell Free Synthesis of a Protein Array on Gold Surface and Real Time Kinetic Monitoring by SPR Imaging

The novel method Nucleic Acid Programmable Protein Array (NAPPA) overcomes limitations by synthesizing the proteins in situ by spotting plasmid DNA encoding the relevant proteins, which are then simultaneously transcribed and translated by a cell free system.

A Microwell-Printing Fabrication Strategy for the On-Chip Templated Biosynthesis of Protein Microarrays for Surface Plasmon Resonance Imaging.

This greatly simplified two-step synthesis/printing fabrication methodology eliminates most of the handling, purification and processing steps normally required in the synthesis of multiple protein probes, and enables the rapid fabrication of SPRI protein microarrays from DNA templates for the study of protein-protein bioaffinity interactions.

Cell-free protein expression and functional assay in nanowell chip format.

The combination of nanowell chip technology and cell-free transcription and translation of proteins is shown and an enzymatic inhibition assay in nanowells is presented to anticipate further applications, such as the high-throughput screening of drug candidates or the identification of novel enzymes for biotechnology.

Self-Assembling Protein Microarrays

This work generated protein microarrays by printing complementary DNAs onto glass slides and then translating target proteins with mammalian reticulocyte lysate, and used the technology to map pairwise interactions among 29 human DNA replication initiation proteins, recapitulate the regulation of Cdt1 binding to select replication proteins, and map its geminin-binding domain.

HaloTag: a novel protein labeling technology for cell imaging and protein analysis.

The utility of this modular protein tagging system for cellular imaging and protein immobilization is demonstrated by analyzing multiple molecular processes associated with NF-kappaB-mediated cellular physiology, including imaging of subcellular protein translocation and capture of protein--protein and protein--DNA complexes.

High density diffusion-free nanowell arrays.

It is determined that diffusion of expressed proteins led to cross-binding at neighboring spots at very high densities with reduced interspot spacing in NAPPA, a robust in situ synthesis method for producing functional proteins just-in-time.

Mapping transcription factor interactome networks using HaloTag protein arrays

The application of HaloTag-NAPPA technology to plant hormone signaling pathways allowed the identification of many novel transcription factor–protein interactions and led to the development of a proteome-wide plant hormone TF interactome network.