Self-Assembling Protein Microarrays

@article{Ramachandran2004SelfAssemblingPM,
  title={Self-Assembling Protein Microarrays},
  author={Niroshan Ramachandran and Eugenie Hainsworth and Bhupinder Singh Bhullar and Samuel Eisenstein and Benjamin Rosen and Albert Y. Lau and Johannes C. Walter and Joshua LaBaer},
  journal={Science},
  year={2004},
  volume={305},
  pages={86 - 90}
}
Protein microarrays provide a powerful tool for the study of protein function. However, they are not widely used, in part because of the challenges in producing proteins to spot on the arrays. We generated protein microarrays by printing complementary DNAs onto glass slides and then translating target proteins with mammalian reticulocyte lysate. Epitope tags fused to the proteins allowed them to be immobilized in situ. This obviated the need to purify proteins, avoided protein stability… Expand
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References

SHOWING 1-10 OF 34 REFERENCES
Printing proteins as microarrays for high-throughput function determination.
TLDR
Miniaturized assays that accommodate extremely low sample volumes and enable the rapid, simultaneous processing of thousands of proteins are developed to facilitate subsequent studies of protein function. Expand
Single step generation of protein arrays from DNA by cell-free expression and in situ immobilisation (PISA method).
TLDR
It is shown that human single-chain antibody fragments and an enzyme (luciferase) can be functionally arrayed by the PISA method, and this method can overcome problems of insolubility or degradation associated with bacterial expression of recombinant proteins. Expand
Recent developments in analytical and functional protein microarrays.
  • G. Jona, M. Snyder
  • Biology, Medicine
  • Current opinion in molecular therapeutics
  • 2003
TLDR
This review discusses the construction of different types of protein arrays, and their numerous and diverse applications for drug discovery and development. Expand
Protein microarrays for gene expression and antibody screening.
TLDR
This application would not be restricted to antigen-antibody systems, protein microarrays should provide a general resource for high-throughput screens of gene expression and receptor-ligand interactions. Expand
Comprehensive Identification of Human bZIP Interactions with Coiled-Coil Arrays
TLDR
Protein arrays used to test pairings of a nearly complete set of coiled-coil strands from human basic-region leucine zipper transcription factors find considerable partnering selectivity despite the bZIPs' homologous sequences. Expand
Proteome-scale purification of human proteins from bacteria
  • P. Braun, Yanhui Hu, +4 authors J. LaBaer
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 2002
TLDR
This work exploited recombinational cloning to facilitate the development of methods for the high-throughput purification of human proteins and observed that some domains in the Pfam database were found almost exclusively in proteins that were successfully purified and thus may have predictive character. Expand
C. elegans ORFeome version 1.1: experimental verification of the genome annotation and resource for proteome-scale protein expression
TLDR
Gateway-clone all predicted protein-encoding open reading frames (ORFs), or the 'ORFeome,' of Caenorhabditis elegans successfully, and it is suggested that similar ORFeome projects will be valuable for other organisms, including humans. Expand
Protein arrays and their role in proteomics.
TLDR
This review outlines recent developments in protein arrays and their applications in proteomics, and discusses the requirements, current limitations and the potential and future perspectives of the technology. Expand
Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells.
TLDR
A comprehensive yeast two-hybrid screen was carried out to establish sequential interactions between two individual proteins of the mouse pre-RC that are probably required for the initiation of DNA replication and revealed multiple interactions among ORC subunits and MCM proteins as well as interactions between individual ORC andMCM proteins. Expand
Assembly of the Human Origin Recognition Complex*
TLDR
The characterization of HsORC should facilitate the identification of human origins of DNA replication and suggest that the subunits HsOrc2, Hs orc3, and Hs Orc4 form a core upon which the ordered assembly of H sOrc5 and HSOrc1 takes place. Expand
...
1
2
3
4
...