Self-Assembling Protein Microarrays

  title={Self-Assembling Protein Microarrays},
  author={Niroshan Ramachandran and E. Hainsworth and B. Bhullar and S. Eisenstein and B. Rosen and A. Lau and J. Walter and J. LaBaer},
  pages={86 - 90}
  • Niroshan Ramachandran, E. Hainsworth, +5 authors J. LaBaer
  • Published 2004
  • Biology, Medicine
  • Science
  • Protein microarrays provide a powerful tool for the study of protein function. However, they are not widely used, in part because of the challenges in producing proteins to spot on the arrays. We generated protein microarrays by printing complementary DNAs onto glass slides and then translating target proteins with mammalian reticulocyte lysate. Epitope tags fused to the proteins allowed them to be immobilized in situ. This obviated the need to purify proteins, avoided protein stability… CONTINUE READING
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