Self-Assembling Protein Microarrays

@article{Ramachandran2004SelfAssemblingPM,
  title={Self-Assembling Protein Microarrays},
  author={Niroshan Ramachandran and E. Hainsworth and B. Bhullar and S. Eisenstein and B. Rosen and A. Lau and J. Walter and J. LaBaer},
  journal={Science},
  year={2004},
  volume={305},
  pages={86 - 90}
}
  • Niroshan Ramachandran, E. Hainsworth, +5 authors J. LaBaer
  • Published 2004
  • Biology, Medicine
  • Science
  • Protein microarrays provide a powerful tool for the study of protein function. However, they are not widely used, in part because of the challenges in producing proteins to spot on the arrays. We generated protein microarrays by printing complementary DNAs onto glass slides and then translating target proteins with mammalian reticulocyte lysate. Epitope tags fused to the proteins allowed them to be immobilized in situ. This obviated the need to purify proteins, avoided protein stability… CONTINUE READING
    510 Citations

    Topics from this paper.

    On-chip protein synthesis for making microarrays.
    • 29
    Producing protein microarrays from DNA microarrays.
    • 5
    Production of protein arrays by cell-free systems.
    • 12
    Preparation of protein arrays using cell-free protein expression.
    • 1
    Protein microarrays printed from DNA microarrays.
    • 4
    Discovering Protein-Protein Interactions Using Nucleic Acid Programmable Protein Arrays.
    • 7
    In situ synthesis of protein arrays.
    • 89
    • PDF
    A cell-free expression platform for production of protein microarrays.
    • 3

    References

    SHOWING 1-10 OF 34 REFERENCES
    Recent developments in analytical and functional protein microarrays.
    • 24
    Proteome-scale purification of human proteins from bacteria
    • P. Braun, Yanhui Hu, +4 authors J. LaBaer
    • Biology, Medicine
    • Proceedings of the National Academy of Sciences of the United States of America
    • 2002
    • 251
    • PDF
    Protein arrays and their role in proteomics.
    • 41
    Assembly of the Human Origin Recognition Complex*
    • 134
    • PDF