Characterization of the Human KCNQ1 Voltage Sensing Domain (VSD) in Lipodisq Nanaoparticles for Electron Paramagnetic Resonance (EPR) Spectroscopic Studies of Membrane Proteins.
@article{Sahu2020CharacterizationOT, title={Characterization of the Human KCNQ1 Voltage Sensing Domain (VSD) in Lipodisq Nanaoparticles for Electron Paramagnetic Resonance (EPR) Spectroscopic Studies of Membrane Proteins.}, author={Indra D. Sahu and Gunjan Dixit and Warren D. Reynolds and Ryan Kaplevatsky and Benjamin D. Harding and Colleen K. Jaycox and Robert M. McCarrick and Gary A. Lorigan}, journal={The journal of physical chemistry. B}, year={2020}, url={https://api.semanticscholar.org/CorpusID:212417320} }
In this work, lipodisq nanoparticles were used as a membrane mimic system for probing the structural and dynamic properties of the more complicated membrane protein system human KCNQ1 voltage sensing domain (Q1-VSD) having four transmembrane helices using site-directed spin labeling EPR spectroscopy.
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Lipodisq nanoparticles were used as a membrane mimic system for probing the structural and dynamic properties of the integral membrane protein KCNE1 using site-directed spin labeling EPR spectroscopy and indicated significant differences in EPR spectral line broadening and a corresponding inverse central line width between spin-labeledKCNE1 residues located inside and outside of the membrane.
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