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Identification of RIP1 kinase as a specific cellular target of necrostatins.
Necroptosis is a cellular mechanism of necrotic cell death induced by apoptotic stimuli in the form of death domain receptor engagement by their respective ligands under conditions where apoptotic execution is prevented and necrostatins are established as the first-in-class inhibitors of RIP1 kinase, the key upstream kinase involved in the activation of necroptosis.
Improved spectral resolution in cosy 1H NMR spectra of proteins via double quantum filtering.
Solution Structure of the Integral Human Membrane Protein VDAC-1 in Detergent Micelles
- S. Hiller, R. Garces, T. Malia, V. Orekhov, M. Colombini, G. Wagner
- Biology, ChemistryScience
- 29 August 2008
NMR measurements revealed the binding sites of VDAC-1 for the BCl-2 protein Bcl-xL, for reduced β–nicotinamide adenine dinucleotide, and for cholesterol as well as voltage-gated channels in phospholipid bilayers similar to those of the native protein.
Optimized phospholipid bilayer nanodiscs facilitate high-resolution structure determination of membrane proteins.
- F. Hagn, M. Etzkorn, Thomas Raschle, G. Wagner
- Biology, ChemistryJournal of the American Chemical Society
- 6 February 2013
This paper demonstrates that the combination of small nanodisc size, high deuteration levels of protein and lipids, and the use of advanced non-uniform NMR sampling methods enable the NMR resonance assignment as well as the high-resolution structure determination of polytopic membrane proteins in a detergent-free, near-native lipid bilayer setting.
Utilization of site-directed spin labeling and high-resolution heteronuclear nuclear magnetic resonance for global fold determination of large proteins with limited nuclear overhauser effect data.
The combination of PBEs with simulated restraints from another nuclear magnetic resonance (NMR) method for global fold determination of large proteins (methyl-protonated, highly deuterated samples) improved the quality of calculated structures.
Amplification of phosphoglycerate dehydrogenase diverts glycolytic flux and contributes to oncogenesis
It is found that in some cancer cells a relatively large amount of glycolytic carbon is diverted into serine and glycine metabolism through phosphoglycerate dehydrogenase (PHGDH).
An ARC/Mediator subunit required for SREBP control of cholesterol and lipid homeostasis
It is shown that SREBPs use the evolutionarily conserved ARC105 (also called MED15) subunit to activate target genes, demonstrating that ARC105 is a key effector of SREBP-dependent gene regulation and control of lipid homeostasis in metazoans.
Solution Structure of BID, an Intracellular Amplifier of Apoptotic Signaling
HIV-1 broadly neutralizing antibody extracts its epitope from a kinked gp41 ectodomain region on the viral membrane.