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Sub‐Micromolar Pulse Dipolar EPR Spectroscopy Reveals Increasing CuII‐labelling of Double‐Histidine Motifs with Lower Temperature
TLDR
The feasibility of exploiting the double‐histidine motif for EPR applications even at sub‐μm protein concentrations in orthogonally labelled CuII–nitroxide systems using a commercial Q‐band EPR instrument is shown. Expand
Prosthetic valve bacterial endocarditis caused by Kingella kingae.
An antibody that prevents serpin polymerisation acts by inducing a novel allosteric behaviour
TLDR
A region potentially exploitable for a rational design of ligands that is able to dynamically influence α1-AT polymerisation is identified, suggesting that prematurely shifting the β-sheet A equilibrium towards the ‘open’ state out of sequence with other changes suppresses polymer formation. Expand
A general model to optimise CuII labelling efficiency of double-histidine motifs for pulse dipolar EPR applications.
TLDR
A general speciation model to optimise CuII labelling efficiency, depending upon pairs of identical or disparate KD values and total CuII label concentration is developed, and the dissociation constant estimates are in excellent agreement with previously determined values. Expand
Pulse Dipolar EPR Reveals Double-Histidine Motif CuII–NTA Spin-Labeling Robustness against Competitor Ions
TLDR
Results demonstrate double-histidine motif spin labeling using CuII-nitrilotriacetic acid (CuII–NTA) is robust against the competitor ligand ZnII-NTA at >1000-fold molar excess, and high nM binding affinity is surprisingly retained under acidic and basic conditions even though room temperature affinity shows a stronger pH dependence. Expand
Nanomolar Pulse Dipolar EPR Spectroscopy in Proteins; the CuII- CuII and Nitroxide-Nitroxide Cases
TLDR
CuII-CuII and nitroxide-nitroxide PDS measurements at protein concentrations below previous examples reaching 500 and 100 nM are demonstrated, demonstrating the general feasibility of sub-μM P DS measurements at short to intermediate distances. Expand
Pricing with Bivariate Unspanned Stochastic Volatility Models
Unspanned stochastic volatility (USV) models have gained popularity in the literature. USV models contain at least one source of volatility-related risk that cannot be hedged with bonds, referred toExpand