An immunogold cell labelling method for rat T lymphocytes

@article{Evans1988AnIC,
  title={An immunogold cell labelling method for rat T lymphocytes},
  author={Gwilym O. Evans and R M Flynn and J D Lupton},
  journal={Laboratory Animals},
  year={1988},
  volume={22},
  pages={332 - 334},
  url={https://api.semanticscholar.org/CorpusID:39961895}
}
A method using monoclonal antibodies in conjunction with an immunogold procedure was adapted for labelling T lymphocytes in blood samples obtained from male Wistar rats. The monoclonal antibodies…
View on SAGE
ncbi.nlm.nih.gov
5 Citations
Methods Citations
1

Figures and Tables from this paper

5 Citations

Cellular and soluble CD4 measurements in cynomolgus monkeys.

Anti-human CD4 monoclonal antibodies have been successfully used to label T-lymphocytes in Cynomolgus monkeys by two different methods and an assay for soluble CD4 has been applied to the sera of these monkeys.

Analytical aspects of FRCS (Fluorescence Activated Cell Sorter) identification of rat T and B peripheral lymphocytes in toxicity studies

There is a strong need for generation and publication of reference values of immunotoxicity parameters within experimental and toxicological areas where the type of distribution of referencevalues is often unknown and will become necessary if current official proposals to implement immune parameters into regulatory toxicity studies are adopted.

Haemopoietic and Lymphatic Systems

III – Haemopoietic and Lymphatic Systems

Chapter 4 – Hemopoietic and Lymphatic Systems

10 References

Immunogold staining: adaptation of a cell-labeling system for analysis of human leukocyte subsets.

Results by IGS and flow cytometry were similar for samples from patients with aberrant lymphocyte populations (e.g., leukemias) or from transplant recipients, suggesting IGS may be a useful diagnostic technique for studying neoplasias or other immunologically mediated disorders.

Immunogold staining method for the light microscopic detection of leukocyte cell surface antigens with monoclonal antibodies: its application to the enumeration of lymphocyte subpopulations.

This immunogold staining method proved to be a reliable tool for the enumeration of T-lymphocyte subpopulations in peripheral blood and can be applied on small volumes of capillary blood.

Monoclonal Antibodies That Define T-Lymphocyte Subsets in the Rat

The possibility that marker antigens have important functional roles is illustrated by the fact that cell-surface immunoglobulin (sIg) is a standard marker for B lymphocytes and is also the receptor for antigen on these cells.

Identification of rat T and B lymphocytes by surface marker analysis

For rats, antithymocyte serum is the method of choice for T cells, whereas SIg determination is the most reliable B-cell marker.

The effects of cyclosporin A on the immune system.

The cyclosporins were originally discovered in 1970 by workers at Sandoz Ltd. in Switzerland who were attempting to identify new antifungal agents but appeared to be selective in its immunosuppressive effects because it had no effect on the survival of mice that had been inoculated with the L1210 leukemia line.

Cyclosporine toxicity in rabbits

Rabbits treated with an immunosuppressive dose of cyclosporine for a prolonged period of time developed a clinically distinct toxic syndrome characterized by wasting, loss of weight, reduced food and water consumption and reduced movement, which was more pronounced in animals given additional indomethacin.

Cyclosporine: a new immunosuppressive agent for organ transplantation.

Lower initial doses of cyclosporine, followed by more rapid tapering may reduce the incidence of nephrotoxicity without compromising improved graft outcome.