dienelactone
National Institutes of Health
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Dienelactone hydrolase, an α/β hydrolase enzyme, catalyzes the hydrolysis of dienelactone to maleylacetate, an intermediate for…
The ease with which enzymes can be adapted from their native roles and engineered to function specifically for industrial or…
Studies of the interactions of dienelactone hydrolase (DLH) and its mutants with both E and Z dienelactone substrates show that…
SummaryStrain RD330 a transposon mutant of Alcaligenes eutrophus JMP134 was considered to be dienelactone hydrolase defective…
A 2, 4-dichlorophenol degrading Pseudomonas strain GT241-1 was isolated from a soil sample. The dienelactone hydrolase gene…
A new and refined method for the purification of DLH has been developed ensuring that this enzyme retains high catalytic activity…
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The structure of DLH (C123S) with PMS bound was solved to 2.5 A resolution (R factor = 15.1%). PMSF in 2-propanol was delivered…
The stereochemistry of the four stereoforms of 5-chloro-3-methylmuconolactones could be deduced from NMR and stability data, and…
Dienelactone hydrolase (EC 3.1.1.45) catalyzes the conversion of cis- or trans-4-carboxymethylenebut-2-en-4-olide (dienelactone…