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Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440.
- K. Nelson, C. Weinel, +40 authors C. Fraser
- Biology, Medicine
- Environmental microbiology
- 1 December 2002
Pseudomonas putida is a metabolically versatile saprophytic soil bacterium that has been certified as a biosafety host for the cloning of foreign genes. The bacterium also has considerable potential… Expand
Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria.
- V. de Lorenzo, M. Herrero, U. Jakubzik, K. Timmis
- Biology, Medicine
- Journal of bacteriology
- 1 November 1990
A collection of Tn5-derived minitransposons has been constructed that simplifies substantially the generation of insertion mutants, in vivo fusions with reporter genes, and the introduction of… Expand
Handbook of hydrocarbon and lipid microbiology
- K. Timmis
VOLUME 1: HYDROCARBONS, OILS AND LIPIDS: DIVERSITY, PROPERTIES AND FORMATION Part 1 Diversity and Physico-Chemical Characteristics, Part 2 Formation and Location, Part 3 Transfer from the Geosphere… Expand
Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria.
A simple procedure for cloning and stable insertion of foreign genes into the chromosomes of gram-negative eubacteria was developed by combining in two sets of plasmids (i) the transposition features… Expand
An evaluation of terminal-restriction fragment length polymorphism (T-RFLP) analysis for the study of microbial community structure and dynamics.
A systematic evaluation of the value and potential of terminal-restriction fragment length polymorphism (T-RFLP) analysis for the study of microbial community structure has been undertaken. The… Expand
Obligate oil-degrading marine bacteria.
Over the past few years, a new and ecophysiologically unusual group of marine hydrocarbon-degrading bacteria - the obligate hydrocarbonoclastic bacteria (OHCB) - has been recognized and shown to play… Expand
Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas.
Host-vector systems have been developed for gene cloning in the metabolically versatile bacterial genus Pseudomonas. They comprise restriction-negative host strains of Pseudomonas aeruginosa and P.… Expand
Alcanivorax borkumensis gen. nov., sp. nov., a new, hydrocarbon-degrading and surfactant-producing marine bacterium.
- M. Yakimov, P. Golyshin, +4 authors K. Timmis
- Medicine, Biology
- International journal of systematic bacteriology
- 1 April 1998
During screening for biosurfactant-producing, n-alkane-degrading marine bacteria, six heterotrophic bacterial strains were isolated from enriched mixed cultures, obtained from sea water/sediment… Expand
Reduction in diversity of the colonic mucosa associated bacterial microflora in patients with active inflammatory bowel disease
Background and aims: The intestinal bacterial microflora plays an important role in the aetiology of inflammatory bowel disease (IBD). As most of the colonic bacteria cannot be identified by culture… Expand