antisecretory factor
National Institutes of Health
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The present invention relates to the use of an antisecretory factor (AF) protein, peptide, derivative, homologue, and/or fragment…
.................................................................................................................................................................... 2 Introduction ............................................................................................................................................................ 4 Theory ....................................................................................................................................................................... 5 Biological Membranes .................................................................................................................................... 5 Liposomes ........................................................................................................................................................... 5 Peptides ................................................................................................................................................................ 5 Antisecretory factor (AF) and the peptide AF16 ................................................................................. 6 Fluorescence Spectroscopy .......................................................................................................................... 6 Tryptophan Emission ..................................................................................................................................... 7 ANTS/DPX Leakage Assay ............................................................................................................................ 7 Isothermal Titration Calorimetry (ITC) ................................................................................................... 8 Linear Dichroism (LD) Spectroscopy ....................................................................................................... 9 Circular Dichroism (CD) Spectroscopy ................................................................................................. 10 Dynamic Light Scattering (DLS) .............................................................................................................. 11 Material and Methods....................................................................................................................................... 11 Material ............................................................................................................................................................. 11 Preparation of Large Unilamellar Vesicles (LUV) ............................................................................. 11 Isothermal Titration Calorimetry (ITC) ................................................................................................ 11 ANTS/DPX Leakage Assay ......................................................................................................................... 12 Linear Dichroism (LD) Spectroscopy .................................................................................................... 12 Circular Dichroism (CD) Spectroscopy ................................................................................................. 13 Dynamic Light Scattering (DLS) .............................................................................................................. 13 Fluorescence Spectroscopy ....................................................................................................................... 13 Results and Discussion .................................................................................................................................... 13 Isothermal Titration Calorimetry (ITC) ................................................................................................ 13 Dynamic Light Scattering (DLS) .............................................................................................................. 16 Circular Dichroism (CD) Spectroscopy ................................................................................................. 17 ANTS/DPX Leakage Assay ......................................................................................................................... 17 Linear Dichroism (LD) Spectroscopy .................................................................................................... 19 Fluorescence Spectroscopy ....................................................................................................................... 20
The present invention relates to the use of an antisecretory factor (AF) protein, peptide, derivative, homologue, and/or fragment…