............................................................................................................................................ 59 Introduction ....................................................................................................................................... 60 Results ............................................................................................................................................... 61 Discussion and outlooks .................................................................................................................... 67 Materials and methods ....................................................................................................................... 71 References ......................................................................................................................................... 74 Acknowledgements ........................................................................................................................... 76 Supporting Material ........................................................................................................................... 76 II CHAPTER 4 .......................................................................................................................................... 81 Resurrection of a Functional Phosphatidylinositol Transfer Protein from a Pseudo-Sec14 Scaffold by Directed Evolution ................................................................................................................................ 81 Abstract ............................................................................................................................................. 81............................................................................................................................................ 8