Skip to search formSkip to main content
You are currently offline. Some features of the site may not work correctly.

Pseudomonas sp. JHN

 
National Institutes of Health

Papers overview

Semantic Scholar uses AI to extract papers important to this topic.
Review
2013
Review
2013
.................................................................................................................................... ii Table of contents ...................................................................................................................... iv List of figures ........................................................................................................................... vi List of tables ............................................................................................................................ vii Acknowledgements ................................................................................................................... x Chapter 1: Introduction ............................................................................................................. 1 1.1 Post-translational modifications ................................................................................................... 1 1.2 O-GlcNAcylation defines a new class of glycoproteins ............................................................... 1 1.3 GlcNAcylation enzymes: OGT and OGA .................................................................................... 4 1.4 Crosstalk between O-GlcNAcylation and phosphorylation ......................................................... 7 1.5 O-GlcNAc and Alzheimer’s disease related protein tau............................................................... 8 1.7 Thesis overview .......................................................................................................................... 12 Chapter 2: Materials and methods .......................................................................................... 15 2.1 Cloning ....................................................................................................................................... 15 2.1.1 His6-SUMO-tau 353-408 ........................................................................................................... 15 2.1.2 Site-directed mutagenesis for aspartic acid mutants ............................................................ 15 2.2 Protein expression and purification ............................................................................................ 17 2.3 NMR spectroscopy ..................................................................................................................... 19 2.4 Aggregation assays ..................................................................................................................... 20 Chapter 3: NMR spectral assignments .................................................................................... 22 3.1 Assignments from main-chain nuclei ......................................................................................... 22 3.2 Cis/trans conformational isomers of tau .................................................................................... 27 3.3 Assignment of O-GlcNAc NMR signals .................................................................................... 28 Chapter 4: Structural, functional, and dynamic studies of tau ................................................ 31 4.1 Chemical shift perturbations ....................................................................................................... 31 4.2 Secondary structure propensities derived from chemical shifts ................................................. 31 4.2.1 δ2D ...................................................................................................................................... 31 4.2.2 3 JHN-Hα coupling .................................................................................................................... 32 4.2.3 NOESY experiments ........................................................................................................... 33 4.3 15 N Relaxation ............................................................................................................................. 37 4.4 Cis/trans isomerization ............................................................................................................... 39 4.5 Aggregation assays ..................................................................................................................... 40 
  • figure 1.1
  • figure 1.2
  • figure 1.3
  • figure 1.4
  • figure 1.5
Is this relevant?
2012
2012
Huntingtonova nemoc (HN) je autozomalně dominantně dědicne neurodegenerativni onemocněni s výskytem cca 1 : 10–15 000. Podstatou… Expand
Is this relevant?