Polik

initiative to promote research collaborations between academia and industry is under threat because universities cannot afford to… (More)

In addition to the general 3'-5' exonuclease domain described by Bernad et al. [Cell 59 (1989) 219-228] significant amino acid… (More)

DNA polymerases normally catalyze DNA synthesis in a template-directed manner. Generally, the continuity of the phosphodiester… (More)

An efficient method for random mutagenesis was applied to a 75-bp target sequence. The mutational changes in the target region… (More)

The hybrid plasmid pRH119 was constructed on the basis of vector plasmid pUC19 and shown to carry Bordetella pertussis PT operon… (More)

For the preparation of gene libraries, DNA from lambda EMBL3 phage was digested with SalI and EcoRI, and the cohesive ends… (More)