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A human P spliceosome structure Splicing of some pre–messenger RNAs could be regulated by cell type–specific splicing factors… Expand The assembly of the spliceosome involves dynamic rearrangements of interactions between snRNAs, protein components, and the pre… Expand The spliceosome is a ribonucleoprotein machine that removes introns from pre-mRNA in a two-step reaction. To investigate the… Expand After the second transesterification step of pre-mRNA splicing, the Prp22 helicase catalyzes release of spliced mRNA by… Expand Slu7 and Prp18 act in concert during the second step of yeast pre-mRNA splicing. Here we show that the 382-amino-acid Slu7… Expand We report that the cyclophilin USA‐CyP is part of distinct complexes with two spliceosomal proteins and is involved in both steps… Expand Pre‐mRNA splicing takes place in two catalytic steps. The second step is poorly understood, especially in mammals. In yeast, the… Expand We show that the requirement for Prp18 during the second step of actin pre-mRNA splicing in vitro is dictated by the distance… Expand We have identified a human splicing factor required for the second step of pre-mRNA splicing. This new protein, hPrp18, is 30… Expand We have analyzed pre-mRNA splicing in yeast extracts immunodepleted of the PRP18 protein. We find that while the first step of… Expand